ArticleNucleic acids research2014
Multiplexing clonality: combining RGB marking and genetic barcoding.
Article in Nucleic acids research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Charting single-cell lineages with synthetic and natural barcodes.Nature reviews. Genetics · 2026Review
- Clonal tracking in cancer and metastasis.Cancer metastasis reviews · 2024Review
- DNA Barcoding Technology for Lineage Recording and Tracing to Resolve Cell Fate Determination.Cells · 2023Review
- Quantification of transgene expression in GSH AAVS1 with a novel CRISPR/Cas9-based approach reveals high transcriptional variation.Molecular therapy. Methods & clinical development · 2022Article
- The Reconstitution Dynamics of Cultivated Hematopoietic Stem Cells and Progenitors Is Independent of Age.International journal of molecular sciences · 2022Article
- Multicolor strategies for investigating clonal expansion and tissue plasticity.Cellular and molecular life sciences : CMLS · 2022Review
- LATE-a novel sensitive cell-based assay for the study of CRISPR/Cas9-related long-term adverse treatment effects.Molecular therapy. Methods & clinical development · 2021Article
- Clonal tracking using embedded viral barcoding and high-throughput sequencing.Nature protocols · 2020Article
- Genetic barcoding reveals clonal dominance in iPSC-derived mesenchymal stromal cells.Stem cell research & therapy · 2020Article
- Clones assemble! The clonal complexity of blood during ontogeny and disease.Experimental hematology · 2020Review
- Development of anFuture science OA · 2019Article
- Cytokine Selection of MSC Clones with Different Functionality.Stem cell reports · 2019Article
- Human Teratoma-Derived Hematopoiesis Is a Highly Polyclonal Process Supported by Human Umbilical Vein Endothelial Cells.Stem cell reports · 2018Article
- Multi-color RGB marking enables clonality assessment of liver tumors in a murine xenograft model.Oncotarget · 2017Article
- MAGPIE: Simplifying access and execution of computational models in the life sciences.PLoS computational biology · 2017Article
- DNA Barcoding Reveals Habitual Clonal Dominance of Myeloma Plasma Cells in the Bone Marrow Microenvironment.Neoplasia (New York, N.Y.) · 2017Article
- Multicolor lineage tracing reveals clonal architecture and dynamics in colon cancer.Nature communications · 2017Article
- Measles virus envelope pseudotyped lentiviral vectors transduce quiescent human HSCs at an efficiency without precedent.Blood advances · 2017Article
- Clonal competition in BcrAbl-driven leukemia: how transplantations can accelerate clonal conversion.Molecular cancer · 2017Article
- The Clonal Fate of Live Cells.Molecular therapy. Methods & clinical development · 2017Review
Corrections and comments
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Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
RGB marking and DNA barcoding are two cutting-edge technologies in the field of clonal cell marking. To combine the virtues of both approaches, we equipped LeGO vectors encoding red, green or blue fluorescent proteins with complex DNA barcodes carrying color-specific signatures. For these vectors, we generated highly complex plasmid libraries that were used for the production of barcoded lentiviral vector particles. In proof-of-principle experiments, we used barcoded vectors for RGB marking of cell lines and primary murine hepatocytes. We applied single-cell polymerase chain reaction to decipher barcode signatures of individual RGB-marked cells expressing defined color hues. This enabled us to prove clonal identity of cells with one and the same RGB color. Also, we made use of barcoded vectors to investigate clonal development of leukemia induced by ectopic oncogene expression in murine hematopoietic cells. In conclusion, by combining RGB marking and DNA barcoding, we have established a novel technique for the unambiguous genetic marking of individual cells in the context of normal regeneration as well as malignant outgrowth. Moreover, the introduction of color-specific signatures in barcodes will facilitate studies on the impact of different variables (e.g. vector type, transgenes, culture conditions) in the context of competitive repopulation studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.