ArticleBiology of the cell2014
H2B ubiquitylation modulates spliceosome assembly and function in budding yeast.
Article in Biology of the cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Decoding RNF20: an epigenetic modifier and beyond.Frontiers in cell and developmental biology · 2026Review
- Identification and characterization ofmicroPublication biology · 2026Article
- Truncating the spliceosomal 'rope protein' Prp45 results in Htz1 dependent phenotypes.RNA biology · 2024Article
- Ready, SET, Go: Post-translational regulation of the histone lysine methylation network in budding yeast.The Journal of biological chemistry · 2021Review
- Identification of Histone Modifications Reveals a Role of H2b Monoubiquitination in Transcriptional Regulation ofInternational journal of biological sciences · 2021Article
- The nuclear cap-binding complex as choreographer of gene transcription and pre-mRNA processing.Genes & development · 2020Review
- Nascent RNA and the Coordination of Splicing with Transcription.Cold Spring Harbor perspectives in biology · 2019Review
- Histone 2B monoubiquitination complex integrates transcript elongation with RNA processing at circadian clock and flowering regulators.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Review
- A role for Mog1 in H2Bub1 and H3K4me3 regulation affecting RNAPII transcription and mRNA export.EMBO reports · 2018Article
- Splicing and transcription touch base: co-transcriptional spliceosome assembly and function.Nature reviews. Molecular cell biology · 2017Review
- Novel functions for chromatin dynamics in mRNA biogenesis beyond transcription.Nucleus (Austin, Tex.) · 2017Review
- Perfect timing: splicing and transcription rates in living cells.Wiley interdisciplinary reviews. RNA · 2017Review
- Posttranslational marks control architectural and functional plasticity of the nuclear pore complex basket.The Journal of cell biology · 2016Article
- Histone H3K36 methylation regulates pre-mRNA splicing in Saccharomyces cerevisiae.RNA biology · 2016Article
- Genetic interaction mapping reveals a role for the SWI/SNF nucleosome remodeler in spliceosome activation in fission yeast.PLoS genetics · 2015Article
- Cotranscriptional histone H2B monoubiquitylation is tightly coupled with RNA polymerase II elongation rate.Genome research · 2014Article
- The ubiquitin-selective chaperone Cdc48/p97 associates with Ubx3 to modulate monoubiquitylation of histone H2B.Nucleic acids research · 2014Article
Corrections and comments
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Authors and funding
7 authors at 6 institutions in 2 countries.
Funding
Abstract
background informationCommitment to splicing occurs co-transcriptionally, but a major unanswered question is the extent to which various modifications of chromatin, the template for transcription in vivo, contribute to the regulation of splicing.
resultsHere, we perform genome-wide analyses showing that inhibition of specific marks - H2B ubiquitylation, H3K4 methylation and H3K36 methylation - perturbs splicing in budding yeast, with each modification exerting gene-specific effects. Furthermore, semi-quantitative mass spectrometry on purified nuclear mRNPs and chromatin immunoprecipitation analysis on intron-containing genes indicated that H2B ubiquitylation, but not Set1-, Set2- or Dot1-dependent H3 methylation, stimulates recruitment of the early splicing factors, namely U1 and U2 snRNPs, onto nascent RNAs.
conclusionsThese results suggest that histone modifications impact splicing of distinct subsets of genes using distinct pathways.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.