ArticleAnalytical chemistry2014
Neutron-encoded mass signatures for quantitative top-down proteomics.
Article in Analytical chemistry, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- FLASHQuant: A Fast Algorithm for Proteoform Quantification in Top-Down Proteomics.Analytical chemistry · 2024Article
- Article
- Targeted Quantification of Proteoforms in Complex Samples by Proteoform Reaction Monitoring.Analytical chemistry · 2024Article
- Top-down proteomics.Nature reviews. Methods primers · 2024Article
- Advancements in Oncoproteomics Technologies: Treading toward Translation into Clinical Practice.Proteomes · 2023Review
- Quantitative Top-Down Proteomics in Complex Samples Using Protein-Level Tandem Mass Tag Labeling.Journal of the American Society for Mass Spectrometry · 2021Article
- Comparing Stable Isotope Enrichment by Gas Chromatography with Time-of-Flight, Quadrupole Time-of-Flight, and Quadrupole Mass Spectrometry.Analytical chemistry · 2021Article
- 21-plex DiLeu Isobaric Tags for High-Throughput Quantitative Proteomics.Analytical chemistry · 2020Article
- High-throughput quantitative top-down proteomics.Molecular omics · 2020Review
- Deep Intact Proteoform Characterization in Human Cell Lysate Using High-pH and Low-pH Reversed-Phase Liquid Chromatography.Journal of the American Society for Mass Spectrometry · 2019Article
- Review
- Proteoform Suite: Software for Constructing, Quantifying, and Visualizing Proteoform Families.Journal of proteome research · 2018Article
- Top-Down Proteomics: Ready for Prime Time?Analytical chemistry · 2018Review
- The Role of Electron Transfer Dissociation in Modern Proteomics.Analytical chemistry · 2018Review
- Multiplexed proteome analysis with neutron-encoded stable isotope labeling in cells and mice.Nature protocols · 2018Article
- Mass Defect-Based Dimethyl Pyrimidinyl Ornithine (DiPyrO) Tags for Multiplex Quantitative Proteomics.Analytical chemistry · 2017Article
- Heavy Sugar and Heavy Water Create Tunable Intact Protein Mass Increases for Quantitative Mass Spectrometry in Any Feed and Organism.Analytical chemistry · 2016Article
- NeuCode Proteomics Reveals Bap1 Regulation of Metabolism.Cell reports · 2016Article
- LC-quadrupole/Orbitrap high-resolution mass spectrometry enables stable isotope-resolved simultaneous quantification and ¹³C-isotopic labeling of acyl-coenzyme A thioesters.Analytical and bioanalytical chemistry · 2016Article
- Elucidating Proteoform Families from Proteoform Intact-Mass and Lysine-Count Measurements.Journal of proteome research · 2016Article
Corrections and comments
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Authors and funding
13 authors.
Funding
Abstract
The ability to acquire highly accurate quantitative data is an increasingly important part of any proteomics experiment, whether shotgun or top-down approaches are used. We recently developed a quantitation strategy for peptides based on neutron encoding, or NeuCode SILAC, which uses closely spaced heavy isotope-labeled amino acids and high-resolution mass spectrometry to provide quantitative data. We reasoned that the strategy would also be applicable to intact proteins and could enable robust, multiplexed quantitation for top-down experiments. We used yeast lysate labeled with either (13)C6(15)N2-lysine or (2)H8-lysine, isotopologues of lysine that are spaced 36 mDa apart. Proteins having such close spacing cannot be distinguished during a medium resolution scan, but upon acquiring a high-resolution scan, the two forms of the protein with each amino acid are resolved and the quantitative information revealed. An additional benefit NeuCode SILAC provides for top down is that the spacing of the isotope peaks indicates the number of lysines present in the protein, information that aids in identification. We used NeuCode SILAC to quantify several hundred isotope distributions, manually identify and quantify proteins from 1:1, 3:1, and 5:1 mixed ratios, and demonstrate MS(2)-based quantitation using ETD.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.