ArticlePlant methods2014
EMS mutagenesis in mature seed-derived rice calli as a new method for rapidly obtaining TILLING mutant populations.
Article in Plant methods, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- Ethyl methanesulfonate mutagenesis in fungi: genetic mechanisms, applications, and implications for agricultural biotechnology.Archives of microbiology · 2026Review
- Chemical-induced variations in callus regeneratedBiotechnology reports (Amsterdam, Netherlands) · 2025Article
- Characterization ofMicroorganisms · 2025Article
- Watermelon fruit metabolome gene discovery and its application in breeding: a review.Frontiers in plant science · 2025Review
- Pheno- and genotyping in vitro dauer juvenile recovery in the nematode Heterorhabditis bacteriophora.Applied microbiology and biotechnology · 2023Article
- Diversity and Abundance of Bacterial and Fungal Communities InhabitingMicroorganisms · 2023Article
- Enhancing the quality of staple food crops through CRISPR/Cas-mediated site-directed mutagenesis.Planta · 2023Review
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- Genome-wide analysis of mutations induced by carbon ion beam irradiation in cotton.Frontiers in plant science · 2023Article
- Mutagenic effects of sodium azide on in vitro mutagenesis, polymorphism and genomic instability in wheat (Triticum aestivum L.).Molecular biology reports · 2022Article
- Ethyl Methane Sulfonate and Sodium Azide-Mediated Chemical and X-ray-Mediated Physical Mutagenesis Positively RegulatePlants (Basel, Switzerland) · 2022Article
- Mutation in BrGGL7 gene encoding a GDSL esterase / lipase causes male sterility in Chinese cabbage (Brassica rapa L. ssp. pekinensis).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- Applications of In Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat.Plants (Basel, Switzerland) · 2022Review
- Morphological, molecular and phytochemical variations induced by colchicine and EMS chemical mutagens inFood chemistry. Molecular sciences · 2022Article
- A Temporary Immersion System Improves Regeneration of In Vitro Irradiated Recalcitrant Indica Rice (Plants (Basel, Switzerland) · 2022Article
- Evaluation of the Saline-Alkaline Tolerance of Rice (Biology · 2022Article
- Article
- Induced variations of ethyl methane sulfonate mutagenized cowpea (Frontiers in plant science · 2022Article
- Optimized methods for random and targeted mutagenesis in field pea (Frontiers in plant science · 2022Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTILLING (Targeting Induced Local Lesions IN Genomes) is a reverse genetic method that combines chemical mutagenesis with high-throughput genome-wide screening for point mutation detection in genes of interest. However, this mutation discovery approach faces a particular problem which is how to obtain a mutant population with a sufficiently high mutation density. Furthermore, plant mutagenesis protocols require two successive generations (M1, M2) for mutation fixation to occur before the analysis of the genotype can begin.
resultsHere, we describe a new TILLING approach for rice based on ethyl methanesulfonate (EMS) mutagenesis of mature seed-derived calli and direct screening of in vitro regenerated plants. A high mutagenesis rate was obtained (i.e. one mutation in every 451 Kb) when plants were screened for two senescence-related genes. Screening was carried out in 2400 individuals from a mutant population of 6912. Seven sense change mutations out of 15 point mutations were identified.
conclusionsThis new strategy represents a significant advantage in terms of time-savings (i.e. more than eight months), greenhouse space and work during the generation of mutant plant populations. Furthermore, this effective chemical mutagenesis protocol ensures high mutagenesis rates thereby saving in waste removal costs and the total amount of mutagen needed thanks to the mutagenesis volume reduction.
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