ArticleJournal of nephropathology2012
Detection of immunoglobulins and complement components in formalin fixed and paraffin embedded renal biopsy material by immunoflourescence technique.
Article in Journal of nephropathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 20 citations in OpenAlex.
- Illuminating Blurry Vision: Visualization of Corneal Protein Deposition With Immunofluorescence in Two Illustrative Case Reports.Case reports in pathology · 2026Article
- A Comparative Study of Immunofluorescence on Formalin-Fixed, Paraffin-Embedded Versus Fresh Frozen Kidney Biopsy.Cureus · 2023Article
- Diagnostic Accuracy of Direct Immunofluorescence Test on Paraffin-Embedded Blocks in Comparison with Frozen Section Blocks in Renal Biopsies.International journal of nephrology · 2022Article
- Fluorescent Immunohistochemistry.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Paraffin Immunofluorescence: A Valuable Ancillary Technique in Renal Pathology.Kidney international reports · 2018Review
- Paraffin immunofluorescence for detection of immune complexes in renal biopsies: an efficient salvage technique for diagnosis of glomerulonephritis in dogs.BMC veterinary research · 2017Article
- Immunofluorescence on paraffin embedded renal biopsies: Experience of a tertiary care center with review of literature.World journal of nephrology · 2016Article
- Resolution of C1q deposition but not of the clinical nephrotic syndrome after immunomodulating therapy in focal sclerosis.Journal of nephropathology · 2015Article
- Significance of extracapillary proliferation in IgA-nephropathy patients with regard to clinical and histopathological variables.Hippokratia · 2013Article
- Anti-phospholipase A2 receptor antibody in idiopathic membranous nephropathy: New concepts.Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences · 2013Article
- Association of proteinuria with various clinical findings and morphologic variables of oxford classification in immunoglobulin a nephropathy patients.International journal of preventive medicine · 2013Article
- Immunoglobulin A Nephropathy and Malaria falciparum Infection; a Rare Association.Iranian journal of public health · 2013Article
- Immunoperoxidase and Immunofluorescence on Formalin-Fixed, Paraffin-Embedded Tissue Sections versus Immunofluorescence on Frozen Sections in the Assessment of Renal Biopsies.Indian journal of nephrologyArticle
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Authors and funding
4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe technique of direct immunoflourescence (IF) is essential in the accurate diagnosis of renal glomerular diseases. The optimal results are obtained when the procedure is done on fresh frozen tissue (IF-F). However, techniques are available for IF study on formalin fixed and paraffin embedded (FFPE) renal biopsy specimens with variable reported success rates.
objectivesWe evaluated three such techniques on FFPE tissue and compared the results with those obtained by IF-F from the same patients. MATERIALS AND
methodsHeat treatment with Tris buffer and citrate buffer, and pronase treatment of the FFPE material was carried out. Direct IF was done for renal panel immunoglobulins and complement components on all biopsies and the results were compared with the historical IF-F study.
resultsWhen compared to the IF-F, the immunoflourescence staining on the paraffin sections was less sensitive and less intense in all immune complex-mediated renal diseases, but the diagnostic findings were detected in majority of the cases.
conclusionsIn conclusion, it is possible to establish the diagnosis in most cases of immune complex-mediated glomerular diseases with IF on paraffin embedded tissue specimens.
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