ArticleIranian journal of microbiology2013
Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran.
Article in Iranian journal of microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 2 of them syntheses that pooled it.
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Who cites it
13 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Pertussis resurgence in iran: A systematic review and meta-analysis of seroimmunity and diagnostic evidence.BMC infectious diseases · 2025Pooled it
- Pooled it
- Automatic Identification of MALDI-TOF MS Database Using ClassicalComputational and mathematical methods in medicine · 2022Article
- Comparison of Two Different Methods for the Extraction of Outer Membrane Vesicles from theArchives of Razi Institute · 2021Article
- Alarm Thresholds for Pertussis Outbreaks in Iran: National Data Analysis.Osong public health and research perspectives · 2020Article
- Ellipsometric-based novel DNA biosensor for label-free, real-time detection of Bordetella parapertussis.Journal of biological physics · 2019Article
- Molecular detection ofIranian journal of microbiology · 2017Article
- Epidemiology of pertussis in Denmark, 1995 to 2013.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2016Article
- Old Disease and New Challenges: Major Obstacles of Current Strategies in the Prevention of Pertussis.Iranian journal of pediatrics · 2016Review
- High prevalence of Bordetella pertussis in children under 5 years old hospitalized with acute respiratory infections in Lima, Peru.BMC infectious diseases · 2015Article
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Virulence Factors Variation Among Bordetella Pertussis Isolates in Iran.International journal of molecular and cellular medicine · 2015Article
- The First Macrolide-Resistant Bordetella pertussis Strains Isolated From Iranian Patients.Jundishapur journal of microbiology · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND AND
objectiveDue to contagiousness of pertussis, a rapid and sensitive method for diagnosis is required to initiate the treatment and interrupt its transmission. MATERIALS AND
methodsTo detect B. pertussis strains, we used two real-time PCR targeting IS481 and BP283 sequences and compared factors influencing culture and real-time PCR results.
resultsTotally, 779 specimens were collected from patients among which 11 (1.4%) were culture positive. Using IS481 and BP283 primers, 122 (15.6%) and 100 (12.8%) were diagnosed as infected specimens respectively. There were significant relationships between the real-time PCR method for diagnosis of B. pertussis and age, sex and vaccination of patients before sampling.
conclusionThe real-time PCR is superior and much more sensitive than culture for diagnosis of B. pertussis. However, the sensitivity was improved when both IS481 and BP283 were used. Correct sampling and transportation of specimen also improved the detection rate in our research.
Indexed as
Identifiers
24475325PMC3895556What OpenQuestion holds
Registered trials
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