ArticlePloS one2014
Structural characterization of the mechanosensitive channel candidate MCA2 from Arabidopsis thaliana.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- MID1-COMPLEMENTING ACTIVITY regulates cell proliferation and development via Ca2+ signaling in Marchantia polymorpha.Plant physiology · 2024Article
- Physiological roles of Arabidopsis MCA1 and MCA2 based on their dynamic expression patterns.Journal of plant research · 2024Review
- Signals and Their Perception for Remodelling, Adjustment and Repair of the Plant Cell Wall.International journal of molecular sciences · 2023Review
- The calcium connection: exploring the intricacies of calcium signaling in plant-microbe interactions.Frontiers in plant science · 2023Review
- Structure, Function, and Applications of Soybean Calcium Transporters.International journal of molecular sciences · 2022Review
- Entanglement of Arabidopsis Seedlings to a Mesh Substrate under Microgravity Conditions in KIBO on the ISS.Plants (Basel, Switzerland) · 2022Article
- Delineating Calcium Signaling Machinery in Plants: Tapping the Potential through Functional Genomics.Current genomics · 2021Review
- MCAs in Arabidopsis are CaNature communications · 2021Article
- Between Stress and Response: Function and Localization of Mechanosensitive CaInternational journal of molecular sciences · 2021Review
- With an Ear Up against the Wall: An Update on Mechanoperception inPlants (Basel, Switzerland) · 2021Review
- PIEZO ion channel is required for root mechanotransduction inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- The gravistimulation-induced very slow CaScientific reports · 2021Article
- Calcium Signaling-Mediated Plant Response to Cold Stress.International journal of molecular sciences · 2018Review
- CaScientific reports · 2018Article
- Plant mechanosensitive ion channels: an ocean of possibilities.Current opinion in plant biology · 2017Review
- Transmembrane Topologies of Ca2+-permeable Mechanosensitive Channels MCA1 and MCA2 in Arabidopsis thaliana.The Journal of biological chemistry · 2015Article
- Single-particle cryo-EM of the ryanodine receptor channel in an aqueous environment.European journal of translational myology · 2015Article
- Plant signaling networks involving Ca(2+) and Rboh/Nox-mediated ROS production under salinity stress.Frontiers in plant science · 2015Review
- United in diversity: mechanosensitive ion channels in plants.Annual review of plant biology · 2015Review
- Mechanosensitive channel candidate MCA2 is involved in touch-induced root responses in Arabidopsis.Frontiers in plant science · 2014Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mechanosensing in plants is thought to be governed by sensory complexes containing a Ca²⁺-permeable, mechanosensitive channel. The plasma membrane protein MCA1 and its paralog MCA2 from Arabidopsis thaliana are involved in mechanical stress-induced Ca²⁺ influx and are thus considered as candidates for such channels or their regulators. Both MCA1 and MCA2 were functionally expressed in Sf9 cells using a baculovirus system in order to elucidate their molecular natures. Because of the abundance of protein in these cells, MCA2 was chosen for purification. Purified MCA2 in a detergent-solubilized state formed a tetramer, which was confirmed by chemical cross-linking. Single-particle analysis of cryo-electron microscope images was performed to depict the overall shape of the purified protein. The three-dimensional structure of MCA2 was reconstructed at a resolution of 26 Å from 5,500 particles and appears to comprise a small transmembrane region and large cytoplasmic region.
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