ArticlePloS one2014
Optimization of DNA recovery and amplification from non-carbonized archaeobotanical remains.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed, 78 citations in OpenAlex.
- Optimizing ancient DNA recovery from archaeological plant seeds.Scientific reports · 2025Article
- Article
- Ancient DNA from a lost Negev Highlands desert grape reveals a Late Antiquity wine lineage.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Morphotype broadening of the grapevine (Vitis vinifera L.) from Oxus civilization 4000 BP, Central Asia.Scientific reports · 2022Article
- Genome Sequencing of up to 6,000-Year-Old Citrullus Seeds Reveals Use of a Bitter-Fleshed Species Prior to Watermelon Domestication.Molecular biology and evolution · 2022Article
- The untapped potential of macrofossils in ancient plant DNA research.The New phytologist · 2022Article
- Molecular Clocks and Archeogenomics of a Late Period Egyptian Date Palm Leaf Reveal Introgression from Wild Relatives and Add Timestamps on the Domestication.Molecular biology and evolution · 2021Article
- Back to the Origins: Background and Perspectives of Grapevine Domestication.International journal of molecular sciences · 2021Review
- Editorial: Integrative and Translational Uses of Herbarium Collections Across Time, Space, and Species.Frontiers in plant science · 2020Article
- Mitochondrial genome evolution in parasitic plants.BMC evolutionary biology · 2019Article
- Paleogenomics: reconstruction of plant evolutionary trajectories from modern and ancient DNA.Genome biology · 2019Article
- Ancient DNA reveals the timing and persistence of organellar genetic bottlenecks over 3,000 years of sunflower domestication and improvement.Evolutionary applications · 2019Article
- High-Throughput DNA sequencing of ancient wood.Molecular ecology · 2018Article
- Development of a Method to Extract Opium Poppy (Papaver somniferum L.) DNA from Heroin.Scientific reports · 2018Article
- Genome Sequencing of Ancient Plant Remains: Findings, Uses and Potential Applications for the Study and Improvement of Modern Crops.Frontiers in plant science · 2018Review
- Comparative performance of the BGISEQ-500 vs Illumina HiSeq2500 sequencing platforms for palaeogenomic sequencing.GigaScience · 2017Article
- Dating the beginning of the Roman viticultural model in the Western Mediterranean: The case study of Chianti (Central Italy).PloS one · 2017Article
- The efficacy of high-throughput sequencing and target enrichment on charred archaeobotanical remains.Scientific reports · 2016Article
- Article
- Successful enrichment and recovery of whole mitochondrial genomes from ancient human dental calculus.American journal of physical anthropology · 2016Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ancient DNA (aDNA) recovered from archaeobotanical remains can provide key insights into many prominent archaeological research questions, including processes of domestication, past subsistence strategies, and human interactions with the environment. However, it is often difficult to isolate aDNA from ancient plant materials, and furthermore, such DNA extracts frequently contain inhibitory substances that preclude successful PCR amplification. In the age of high-throughput sequencing, this problem is even more significant because each additional endogenous aDNA molecule improves analytical resolution. Therefore, in this paper, we compare a variety of DNA extraction techniques on primarily desiccated archaeobotanical remains and identify which method consistently yields the greatest amount of purified DNA. In addition, we test five DNA polymerases to determine how well they replicate DNA extracted from non-charred ancient plant remains. Based upon the criteria of resistance to enzymatic inhibition, behavior in quantitative real-time PCR, replication fidelity, and compatibility with aDNA damage, we conclude these polymerases have nuanced properties, requiring researchers to make educated decisions as to which one to use for a given task. The experimental findings should prove useful to the aDNA and archaeological communities by guiding future research methodologies and ensuring precious archaeobotanical remains are studied in optimal ways, and may thereby yield important new perspectives on the interactions between humans and past plant communities.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.