ArticlePloS one2014
In vivo Pyro-SIP assessing active gut microbiota of the cotton leafworm, Spodoptera littoralis.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
46 citing papers in PubMed, 103 citations in OpenAlex.
- Structure of the Gut and Ovary, with Associated Microbiota Across Life Stages in the Striped Stem BorerInsects · 2026Article
- Metagenomic Analysis of Gut Microbiome Across Developmental Stage of Asian Corn Borer (Insects · 2026Article
- Composition and Diversity Characteristics of Gut Microbiota during the Development ofEcology and evolution · 2026Article
- Comparative Effects ofInternational journal of molecular sciences · 2026Article
- Temperature dependent immunological responses of Spoladea recurvalis exposed to entomopathogenic fungi.Scientific reports · 2026Article
- Diversity of culturable gut bacteria associated with brown planthopper, Nilaparvata lugens (Stål) and their role in imidacloprid degradation.Scientific reports · 2026Article
- Investigation of probiotic, anticancer and antimicrobial activity of Enterococcus bacteria isolated from the gut microbiota of marine snails.International microbiology : the official journal of the Spanish Society for Microbiology · 2026Article
- Life inside a bag: multiomics insights into the bagworm species Eumeta crameri.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025Article
- Assessing gut microbiota diversity and functional potential in resistant and susceptible strains of the mediterranean fruit fly.Scientific reports · 2025Article
- Host Diet and Species Interact to Shape the Bacterial and Fungal Microbiome in the Regurgitant of Four Spodoptera Species.Microbial ecology · 2025Article
- Effect ofInsects · 2024Article
- Sortase-encoding genes,Frontiers in microbiology · 2024Article
- Article
- Article
- Article
- Article
- A soil fungus confers plant resistance against a phytophagous insect by disrupting the symbiotic role of its gut microbiota.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Microbial Community Structure among Honey Samples of Different Pollen Origin.Antibiotics (Basel, Switzerland) · 2023Article
- Microbiome analysis ofPeerJ · 2023Article
- Impact of transgenerational host switch on gut bacterial assemblage in generalist pest,Frontiers in microbiology · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The gut microbiota is of crucial importance for the host with considerable metabolic activity. Although great efforts have been made toward characterizing microbial diversity, measuring components' metabolic activity surprisingly hasn't kept pace. Here we combined pyrosequencing of amplified 16S rRNA genes with in vivo stable isotope probing (Pyro-SIP) to unmask metabolically active bacteria in the gut of cotton leafworm (Spodoptera littoralis), a polyphagous insect herbivore that consumes large amounts of plant material in a short time, liberating abundant glucose in the alimentary canal as a most important carbon and energy source for both host and active gut bacteria. With (13)C glucose as the trophic link, Pyro-SIP revealed that a relatively simple but distinctive gut microbiota co-developed with the host, both metabolic activity and composition shifting throughout larval stages. Pantoea, Citrobacter and Clostridium were particularly active in early-instar, likely the core functional populations linked to nutritional upgrading. Enterococcus was the single predominant genus in the community, and it was essentially stable and metabolically active in the larval lifespan. Based on that Enterococci formed biofilm-like layers on the gut epithelium and that the isolated strains showed antimicrobial properties, Enterococcus may be able to establish a colonization resistance effect in the gut against potentially harmful microbes from outside. Not only does this establish the first in-depth inventory of the gut microbiota of a model organism from the mostly phytophagous Lepidoptera, but this pilot study shows that Pyro-SIP can rapidly gain insight into the gut microbiota's metabolic activity with high resolution and high precision.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.