ArticleProceedings of the National Academy of Sciences of the United States of America2014
A variable homopolymeric G-repeat defines small RNA-mediated posttranscriptional regulation of a chemotaxis receptor in Helicobacter pylori.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 77 citations in OpenAlex.
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- Conserved mycobacterial sRNA B11 regulates lipooligosaccharide synthesis at posttranscriptional level inmLife · 2025Article
- DNA uptake and twitching motility are controlled by the small RNA Arp through repression of pilin translation inbioRxiv : the preprint server for biology · 2025Article
- Kastor: a reference-based comparative approach for assessment and correction of gene-fragmenting errors in long-read assemblies of small genomes.BMC genomics · 2025Article
- An RNase III-processed sRNA coordinates sialic acid metabolism ofProceedings of the National Academy of Sciences of the United States of America · 2025Article
- An sRNA overexpression library reveals AbnZ as a negative regulator of an essential translocation module in Caulobacter crescentus.Nucleic acids research · 2025Article
- Interplay of two small RNAs fine-tunes hierarchical flagella gene expression in Campylobacter jejuni.Nature communications · 2024Article
- Molecular insights into the fine-tuning of pH-dependent ArsR-mediated regulation of the SabA adhesin in Helicobacter pylori.Nucleic acids research · 2024Article
- Review
- Article
- Role of nickel-regulated small RNA in modulation ofWorld journal of clinical cases · 2022Review
- Helicobacter pylori shows tropism to gastric differentiated pit cells dependent on urea chemotaxis.Nature communications · 2022Article
- Posttranscriptional Regulation in Response to Different Environmental Stresses in Campylobacter jejuni.Microbiology spectrum · 2022Article
- Spacer prioritization in CRISPR-Cas9 immunity is enabled by the leader RNA.Nature microbiology · 2022Article
- Comparative Genome Analysis Reveals Accumulation of Single-Nucleotide Repeats in PathogenicCurrent issues in molecular biology · 2022Article
- RNase III-mediated processing of aeLife · 2021Article
- Small RNA mediated gradual control of lipopolysaccharide biosynthesis affects antibiotic resistance in Helicobacter pylori.Nature communications · 2021Article
- A bacterial small RNA regulates the adaptation of Helicobacter pylori to the host environment.Nature communications · 2021Article
- Riboregulation in the Major Gastric PathogenFrontiers in microbiology · 2021Review
- Diversity and Versatility in Small RNA-Mediated Regulation in Bacterial Pathogens.Frontiers in microbiology · 2021Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phase variation of hypermutable simple sequence repeats (SSRs) is a widespread and stochastic mechanism to generate phenotypic variation within a population and thereby contributes to host adaptation of bacterial pathogens. Although several examples of SSRs that affect transcription or coding potential have been reported, we now show that a SSR also impacts small RNA-mediated posttranscriptional regulation. Based on in vitro and in vivo analyses, we demonstrate that a variable homopolymeric G-repeat in the leader of the TlpB chemotaxis receptor mRNA of the human pathogen Helicobacter pylori is directly targeted by a small RNA (sRNA), RepG (Regulator of polymeric G-repeats). Whereas RepG sRNA is highly conserved, the tlpB G-repeat length varies among diverse H. pylori strains, resulting in strain-specific RepG-mediated tlpB regulation. Based on modification of the G-repeat length within one strain, we demonstrate that the G-repeat length determines posttranscriptional regulation and can mediate both repression and activation of tlpB through RepG. In vitro translation assays show that this regulation occurs at the translational level and that RepG influences tlpB translation dependent on the G-repeat length. In contrast to the digital ON-OFF switches through frame-shift mutations within coding sequences, such modulation of posttranscriptional regulation allows for a gradual control of gene expression. This connection to sRNA-mediated posttranscriptional regulation might also apply to other genes with SSRs, which could be targeting sites of cis- or trans-encoded sRNAs, and thereby could facilitate host adaptation through sRNA-mediated fine-tuning of virulence gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.