ArticleProceedings of the National Academy of Sciences of the United States of America2014
Transcriptional occlusion caused by overlapping promoters.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- DksA-Dependent Stringent Stress Response Drives Virulence and Gastrointestinal Persistence ofbioRxiv : the preprint server for biology · 2026Article
- Klebsiella pneumoniae employs a type VI secretion system to overcome microbiota-mediated colonization resistance.Nature communications · 2025Article
- RpoS and the bacterial general stress response.Microbiology and molecular biology reviews : MMBR · 2024Review
- RpoS-Regulated Genes and Phenotypes in the Phytopathogenic BacteriumInternational journal of molecular sciences · 2023Article
- Single-Cell Heterogeneity in Snake Venom Expression Is Hardwired by Co-Option of Regulators from Progressively Activated Pathways.Genome biology and evolution · 2023Article
- BosR and PlzA reciprocally regulate RpoS function to sustain Borrelia burgdorferi in ticks and mammals.The Journal of clinical investigation · 2023Article
- Klebsiella pneumoniae l-Fucose Metabolism Promotes Gastrointestinal Colonization and Modulates Its Virulence Determinants.Infection and immunity · 2022Article
- Genome-wide promoter assembly inGenome research · 2022Article
- Regulatory Small RNA Qrr2 Is Expressed Independently of Sigma Factor-54 and Can Function as the Sole Qrr Small RNA To Control Quorum Sensing in Vibrio parahaemolyticus.Journal of bacteriology · 2022Article
- σFrontiers in microbiology · 2021Article
- Tunable Transcriptional Interference at the Endogenous Alcohol Dehydrogenase Gene Locus inG3 (Bethesda, Md.) · 2020Article
- Construction and Characterization of a Medium Copy Number Expression Vector Carrying Auto-Inducible dps Promoter to Overproduce a Bacterial Superoxide Dismutase in Escherichia coli.Molecular biotechnology · 2019Article
- Small RNAs Involved in Regulation of Nitrogen Metabolism.Microbiology spectrum · 2018Review
- Genome-Wide Transcriptional Response to Varying RpoS Levels in Escherichia coli K-12.Journal of bacteriology · 2017Article
- Envelope Stress Responses: An Interconnected Safety Net.Trends in biochemical sciences · 2017Review
- Transcription-coupled changes to chromatin underpin gene silencing by transcriptional interference.Nucleic acids research · 2016Article
- Protection against deleterious nitrogen compounds: role of σS-dependent small RNAs encoded adjacent to sdiA.Nucleic acids research · 2016Article
- Characterization of acetic acid-detoxifying Escherichia coli evolved under phosphate starvation conditions.Microbial cell factories · 2016Article
- The Emergence of 2-Oxoglutarate as a Master Regulator Metabolite.Microbiology and molecular biology reviews : MMBR · 2015Review
- Genome-Scale Mapping of Escherichia coli σ54 Reveals Widespread, Conserved Intragenic Binding.PLoS genetics · 2015Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
RpoS (σ(38)) is required for cell survival under stress conditions, but it can inhibit growth if produced inappropriately and, consequently, its production and activity are elaborately regulated. Crl, a transcriptional activator that does not bind DNA, enhances RpoS activity by stimulating the interaction between RpoS and the core polymerase. The crl gene has two overlapping promoters, a housekeeping, RpoD- (σ(70)) dependent promoter, and an RpoN (σ(54)) promoter that is strongly up-regulated under nitrogen limitation. However, transcription from the RpoN promoter prevents transcription from the RpoD promoter, and the RpoN-dependent transcript lacks a ribosome-binding site. Thus, activation of the RpoN promoter produces a long noncoding RNA that silences crl gene expression simply by being made. This elegant and economical mechanism, which allows a near-instantaneous reduction in Crl synthesis without the need for transacting regulatory factors, restrains the activity of RpoS to allow faster growth under nitrogen-limiting conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.