ArticleThe Plant cell2014
NITROGEN LIMITATION ADAPTATION recruits PHOSPHATE2 to target the phosphate transporter PT2 for degradation during the regulation of Arabidopsis phosphate homeostasis.
Article in The Plant cell, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 115 papers.
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Who cites it
115 citing papers in PubMed, 320 citations in OpenAlex.
- Active ROP2 triggers leaf senescence and orchestrates the growth-senescence trade-off under nitrogen starvation.Plant signaling & behavior · 2026Article
- Hidden conversations beneath the soil: microRNA and hormone control of root symbioses.Archives of microbiology · 2026Review
- The PHO1 protein family in phosphate export and beyond.Plant physiology · 2026Review
- ZmPHR1 and ZmPHR2 Mediate Metabolic and Microbial Regulation of Maize Adaptation to Phosphorus Heterogeneity.Plant, cell & environment · 2026Article
- The SPX protein family in plants: from phosphate sensors to multifunctional signaling hubs.Stress biology · 2026Review
- Article
- Medicago phosphate exporter PHO1.3 regulates arbuscular mycorrhizal symbiosis.BMC plant biology · 2026Article
- Adaptation mechanisms of low-phosphorus stress in plants: physiological responses, molecular regulation, and future perspectives.Frontiers in plant science · 2026Review
- Overexpression of miR399d impairs arbuscular mycorrhizal symbiosis in tomato.Plant biotechnology journal · 2025Article
- Genome-wide identification of wheat Ubiquitin-conjugating enzyme gene family and its role in wheat powdery mildew disease resistance.BMC plant biology · 2025Article
- Effects of suppression of phosphate transporter 4;4 on COJournal of plant research · 2025Article
- Genome-wide identification and analysis of phosphate utilization related genes (PURs) reveal their roles involved in low phosphate responses in Brassica napus L.BMC plant biology · 2025Article
- TaTCP6 is required for efficient and balanced utilization of nitrate and phosphorus in wheat.Nature communications · 2025Article
- Down-regulation of the rice HRS1 HOMOLOG3 transcriptional repressor gene due to N deficiency directly co-activates ammonium and phosphate transporter genes.Journal of experimental botany · 2025Article
- Cross-species analysis of abiotic stress in hydroponic leafy crops reveals conserved regulatory networks and key divergences.Frontiers in plant science · 2025Article
- Molecular Mechanisms of Phosphate Use Efficiency inInternational journal of molecular sciences · 2024Article
- MicroRNAs as potent regulators in nitrogen and phosphorus signaling transduction and their applications.Stress biology · 2024Review
- Mining for QTL controlling maize low-phosphorus response genes combined with deep resequencing of RIL parental genomes and in silico GWAS analysis.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- Mining key genes associated with phosphorus deficiency through genome-wide identification and characterization of cucumber SPX family genes.BMC plant biology · 2024Article
- Untranslated yet indispensable-UTRs act as key regulators in the environmental control of gene expression.Journal of experimental botany · 2024Review
55 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The NITROGEN LIMITATION ADAPTION (NLA) gene was initially shown to function in nitrogen limitation responses; however, recent work shows that the nla mutant hyperaccumulates Pi, phenocopying the Pi signaling mutant pho2. PHO2 encodes a putative E2 conjugase, UBC24. Here, we show that NLA is an E3 ligase that specifically requires UBC24 for polyubiquitination in Arabidopsis thaliana. Among five members of the Pht1 Pi-transporter family tested, NLA associates only with PT2 (Pht1;4). The NLA-UBC24 pair mediates polyubiquitination of PT2 but not PT1. Posttranslational decay of PT2 at high Pi is blocked in pho2 and inhibited by MG132, indicating the requirement of UBC24 and 26S proteasomes. Consistent with NLA/UBC24 function, induced NLA expression causes a UBC24-dependent decrease in PT2 levels. Confocal microscopy of fusion proteins revealed an NLA/PT2 interaction at the plasma membrane. Collectively, these results show that under Pi-replete conditions, NLA and UBC24 target the PT2 transporter for destruction. During the Pi deprivation response, NLA and PHO2 transcripts are cleaved by miR399 and miR827, respectively, and our results suggest that this downregulation relieves the posttranslational repression of PT2, allowing it to accumulate and participate in Pi uptake. Our work provides additional molecular details describing Pi signaling/homeostasis regulation by identifying NLA and UBC24 as partners and PT2 as one of their downstream targets.
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