ArticleVirus genes2014
Inhibiting replication of begomoviruses using artificial zinc finger nucleases that target viral-conserved nucleotide motif.
Article in Virus genes, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- The multidimensional significance of virus-host protein interactions and their implications in the antiviral defense of plants.Stress biology · 2026Review
- Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production.Frontiers in genome editing · 2024Review
- Natural and Engineered Resistance Mechanisms in Plants against Phytoviruses.Pathogens (Basel, Switzerland) · 2023Review
- LbCas12a mediated suppression ofFrontiers in plant science · 2023Article
- Unclasping potentials of genomics and gene editing in chickpea to fight climate change and global hunger threat.Frontiers in genetics · 2023Review
- Advances in S gene targeted genome-editing and its applicability to disease resistance breeding in selectedBioengineered · 2022Article
- Genome Editing and Designer Crops for the Future.Methods in molecular biology (Clifton, N.J.) · 2022Article
- CRISPR/Cas systems versus plant viruses: engineering plant immunity and beyond.Plant physiology · 2021Article
- Genome editing for resistance against plant pests and pathogens.Transgenic research · 2021Review
- Integrating CRISPR-Cas and Next Generation Sequencing in Plant Virology.Frontiers in genetics · 2021Review
- Engineering plant virus resistance: from RNA silencing to genome editing strategies.Plant biotechnology journal · 2020Review
- Control of Plant Viruses by CRISPR/Cas System-Mediated Adaptive Immunity.Frontiers in microbiology · 2020Review
- Exploration of Plant-Microbe Interactions for Sustainable Agriculture in CRISPR Era.Microorganisms · 2019Review
- Genome editing for plant disease resistance: applications and perspectives.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Article
- Harnessing Genome Editing Techniques to Engineer Disease Resistance in Plants.Frontiers in plant science · 2019Review
- Antiviral Defenses in Plants through Genome Editing.Frontiers in microbiology · 2017Review
- Use of designer nucleases for targeted gene and genome editing in plants.Plant biotechnology journal · 2016Review
- Engineering Plant Immunity: Using CRISPR/Cas9 to Generate Virus Resistance.Frontiers in plant science · 2016Review
- Boosting plant immunity with CRISPR/Cas.Genome biology · 2015Review
- Artificial TALE as a Convenient Protein Platform for Engineering Broad-Spectrum Resistance to Begomoviruses.Viruses · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Geminiviridae consists of a large group of single-stranded DNA viruses that cause tremendous losses worldwide. Frequent mixed infection and high rates of recombination and mutation allow them to adapt rapidly to new hosts and overcome hosts' resistances. Therefore, an effective strategy able to confer plants with resistance against multiple begomoviruses is needed. In the present study, artificial zinc finger proteins were designed based on a conserved sequence motif of begomoviruses. DNA-binding affinities and specificities of these artificial zinc fingers were evaluated using electrophoretic mobility shift assay. Artificial zinc finger nuclease (AZFNs) were then constructed based on the ones with the highest DNA-binding affinities. In vitro digest and transient expression assay showed that these AZFNs can efficiently cleave the target sequence and inhibit the replication of different begomoviruses. These results suggest that artificial zinc finger protein technology may be used to achieve resistance against multiple begomoviruses.
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Registered trials
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