Evidence map›Paper›PMID 24473466›Full record

ArticleScientific reports2014

A combinatorial cell-laden gel microarray for inducing osteogenic differentiation of human mesenchymal stem cells.

Alireza Dolatshahi-Pirouz, Mehdi Nikkhah, Akhilesh K Gaharwar, Basma Hashmi, Enrico Guermani, Hamed Aliabadi, Gulden Camci-Unal, Thomas Ferrante, Morten Foss, Donald E Ingber and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.

0numbers the graph read from it
0cells of the map it votes in
44citing papers in PubMed
10.4field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

44 citing papers in PubMed, 140 citations in OpenAlex.

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  14. Self-Healing Hydrogels: The Next Paradigm Shift in Tissue Engineering?Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 2 countries.

Alireza Dolatshahi-Pirouz1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA [2] Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA [3] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA [4] Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark.
Mehdi Nikkhah1] Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA [2] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Akhilesh K Gaharwar1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA [2] Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA [3] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA [4] David H. Koch Institute of Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA [5].
Basma Hashmi1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA [2] School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA [3] Vascular Biology Program, Departments of Pathology and Surgery, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA.
Enrico Guermani1] Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA [2] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Hamed Aliabadi1] Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA [2] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Gulden Camci-Unal1] Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA [2] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Thomas FerranteWyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA.
Morten FossInterdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark.
Donald E Ingber1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA [2] School of Engineering and Applied Sciences, Harvard University, Cambridge, MA 02138, USA [3] Vascular Biology Program, Departments of Pathology and Surgery, Children's Hospital Boston, Harvard Medical School, Boston, MA 02115, USA.
Ali Khademhosseini1] Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA 02115, USA [2] Center for Biomedical Engineering, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA 02139, USA [3] Harvard-MIT Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Harvard University · USAarhus University · DKBrigham and Women's Hospital · US

Funding

Microengineered Osteons for Bone Tissue EngineeringR01AR057837 · NIAMS · UNIVERSITY OF TEXAS HLTH SCI CTR HOUSTON · PI YANG, YUNZHI · 2010 to 2018
$5.1M
Micromechanical Determinants of Organ Design and Engineering (SysCODE 6 of 10)RL1DE019023 · NIDCR · BOSTON CHILDREN'S HOSPITAL · PI INGBER, DONALD E · 2007 to 2011
$2.9M
3D combinatorial microenvironments for effective cell based therapyR01HL099073 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI KHADEMHOSSEINI, ALI, LIAO, RONGLIH · 2010 to 2013
$2.6M
Microfabricated Vascularized 3D Tissue Engineered ConstructsR01HL092836 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI KHADEMHOSSEINI, ALI · 2008 to 2012
$2.4M
Microengineered environments for epithelial-mesenchymal interactions (SysCODE 7RL1DE019024 · NIDCR · BRIGHAM AND WOMEN'S HOSPITAL · PI KHADEMHOSSEINI, ALI · 2007 to 2011
$1.9M
NHLBI NIH HHS HL092836NHLBI NIH HHS HL099073NHLBI NIH HHS R01 HL092836NHLBI NIH HHS R01 HL099073NIAMS NIH HHS AR057837NIAMS NIH HHS R01 AR057837NIDCR NIH HHS DE019023NIDCR NIH HHS DE019024NIDCR NIH HHS RL1 DE019023NIDCR NIH HHS RL1 DE019024
6 · The paper itself

Abstract

Development of three dimensional (3D) microenvironments that direct stem cell differentiation into functional cell types remains a major challenge in the field of regenerative medicine. Here, we describe a new platform to address this challenge by utilizing a robotic microarray spotter for testing stem cell fates inside various miniaturized cell-laden gels in a systematic manner. To demonstrate the feasibility of our platform, we evaluated the osteogenic differentiation of human mesenchymal stem cells (hMSCs) within combinatorial 3D niches. We were able to identify specific combinations, that enhanced the expression of osteogenic markers. Notably, these 'hit' combinations directed hMSCs to form mineralized tissue when conditions were translated to 3D macroscale hydrogels, indicating that the miniaturization of the experimental system did not alter stem cell fate. Overall, our findings confirmed that the 3D cell-laden gel microarray can be used for screening of different conditions in a rapid, cost-effective, and multiplexed manner for a broad range of tissue engineering applications.

Indexed as

Cell DifferentiationCells, CulturedHumansHydrogelsMesenchymal Stem CellsOsteogenesisTissue Array AnalysisTissue EngineeringHydrogels

Identifiers

PMID24473466
PMCPMC3905276
OpenAlexW2068161752

What OpenQuestion holds

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LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.