ReviewMolecules (Basel, Switzerland)2014
Applications of copper-catalyzed click chemistry in activity-based protein profiling.
Review in Molecules (Basel, Switzerland), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 85 citations in OpenAlex.
- Turbocharging crop breeding with integrated biotechnology for a climate-resilient future.Journal of integrative plant biology · 2026Review
- Click chemistry for natural product-inspired covalent drug discovery.Drug discovery today · 2025Review
- TargetingMicrobiology (Reading, England) · 2024Review
- In-silico target prediction by ensemble chemogenomic model based on multi-scale information of chemical structures and protein sequences.Journal of cheminformatics · 2023Article
- Methyltetrazine as a small live-cell compatible bioorthogonal handle for imaging enzyme activitiesRSC chemical biology · 2022Article
- Enhancing Cysteine Chemoproteomic Coverage through Systematic Assessment of Click Chemistry Product Fragmentation.Analytical chemistry · 2022Article
- Profiling sirtuin activity using Copper-free click chemistry.Bioorganic chemistry · 2021Article
- SP3-FAIMS Chemoproteomics for High-Coverage Profiling of the Human Cysteinome*.Chembiochem : a European journal of chemical biology · 2021Article
- Reimagining high-throughput profiling of reactive cysteines for cell-based screening of large electrophile libraries.Nature biotechnology · 2021Article
- Multiplexed CuAAC Suzuki-Miyaura Labeling for Tandem Activity-Based Chemoproteomic Profiling.Analytical chemistry · 2021Article
- Bioorthogonal Reactions in Activity-Based Protein Profiling.Molecules (Basel, Switzerland) · 2020Review
- Covalent allosteric modulation: An emerging strategy for GPCRs drug discovery.European journal of medicinal chemistry · 2020Review
- Rhodium-catalyzed phosphorylation reaction of water-soluble disulfides using hypodiphosphoric acid tetraalkyl esters in water.RSC advances · 2020Article
- Chemical Proteomics and Phenotypic Profiling Identifies the Aryl Hydrocarbon Receptor as a Molecular Target of the Utrophin Modulator Ezutromid.Angewandte Chemie (International ed. in English) · 2020Article
- The clickable activity-based probe of anti-apoptotic calenduloside E.Pharmaceutical biology · 2019Article
- Recent Developments in Cell Permeable Deubiquitinating Enzyme Activity-Based Probes.Frontiers in chemistry · 2019Review
- Graphene-based biosensor for on-chip detection of bio-orthogonally labeled proteins to identify the circulating biomarkers of aging during heterochronic parabiosis.Lab on a chip · 2018Article
- Development of Activity-Based Chemical Probes for Human Sirtuins.ACS chemical biology · 2018Article
- Targets Fishing and Identification of Calenduloside E as Hsp90AB1: Design, Synthesis, and Evaluation of Clickable Activity-Based Probe.Frontiers in pharmacology · 2018Article
- Advanced Activity-Based Protein Profiling Application Strategies for Drug Development.Frontiers in pharmacology · 2018Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Activity-based protein profiling (ABPP) is a chemical proteomic technique that enables the interrogation of protein activity directly within complex proteomes. Given the dominant role of posttranslational modifications in regulating protein function in vivo, ABPP provides a direct readout of activity that is not attained through traditional proteomic methods. ABPP relies on the design of covalent binding probes that either target a specific enzyme or a class of enzymes with related function. These covalent warheads are coupled to either fluorophores or biotin groups for visualization and enrichment of these active proteins. The advent of bioorthogonal chemistries, in particular, the copper (I)-catalyzed azide-alkyne cycloaddition (CuAAC), has benefitted the field of ABPP by achieving the following: (1) replacing bulky reporter groups with smaller alkyne or azide groups to promote cell permeability; (2) adding modularity to the system such that a single probe can be diversified with a variety of reporter groups without the need to develop new synthetic routes; and (3) enabling the conjugation of complex linkers to facilitate quantitative proteomic analyses. Here, we summarize recent examples of CuAAC in ABPP that serve to illustrate the contribution of bioorthogonal chemistry to advancing discoveries in this field.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.