ArticleJournal of visualized experiments : JoVE2014
Cytological analysis of spermatogenesis: live and fixed preparations of Drosophila testes.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Protocol for high-sensitivity detection of microRNAs in adult Drosophila tissues using hybridization chain reaction.STAR protocols · 2026Article
- Peroxisomal DBP deficiency causes male infertility through disruption of lipid homeostasis in Drosophila.Cellular and molecular life sciences : CMLS · 2026Article
- Orthologs of an essential orphan gene vary in their capacities for function and subcellular localization in Drosophila melanogaster.Molecular biology and evolution · 2026Article
- Alternative splicing repurposes the Drosophila mitotic regulator Mud for meiotic functions.Genetics · 2025Article
- Article
- Live Imaging and Analysis of Meiotic Cytokinesis inBio-protocol · 2024Article
- Recovery from heat-induced infertility-A study of reproductive tissue responses and fitness consequences in maleEcology and evolution · 2022Article
- Delivery of ceramide phosphoethanolamine lipids to the cleavage furrow through the endocytic pathway is essential for male meiotic cytokinesis.PLoS biology · 2022Article
- Article
- Strongly TruncatedFrontiers in genetics · 2022Article
- The Drosophila orthologue of the primary ciliary dyskinesia-associated gene, DNAAF3, is required for axonemal dynein assembly.Biology open · 2021Article
- Twitchy, the Drosophila orthologue of the ciliary gating protein FBF1/dyf-19, is required for coordinated locomotion and male fertility.Biology open · 2021Article
- Dissecting Fertility Functions ofGenetics · 2020Article
- Combover interacts with the axonemal component Rsp3 and is required forDevelopment (Cambridge, England) · 2019Article
- Characterization of aBiology open · 2018Article
- Progeny of old parents have increased social space in Drosophila melanogaster.Scientific reports · 2018Article
- Phosphorylation of the RNA-binding protein Dazl by MAPKAP kinase 2 regulates spermatogenesis.Molecular biology of the cell · 2016Article
- CEP63 deficiency promotes p53-dependent microcephaly and reveals a role for the centrosome in meiotic recombination.Nature communications · 2015Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Drosophila melanogaster is a powerful model system that has been widely used to elucidate a variety of biological processes. For example, studies of both the female and male germ lines of Drosophila have contributed greatly to the current understanding of meiosis as well as stem cell biology. Excellent protocols are available in the literature for the isolation and imaging of Drosophila ovaries and testes(3-12). Herein, methods for the dissection and preparation of Drosophila testes for microscopic analysis are described with an accompanying video demonstration. A protocol for isolating testes from the abdomen of adult males and preparing slides of live tissue for analysis by phase-contrast microscopy as well as a protocol for fixing and immunostaining testes for analysis by fluorescence microscopy are presented. These techniques can be applied in the characterization of Drosophila mutants that exhibit defects in spermatogenesis as well as in the visualization of subcellular localizations of proteins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.