ArticleJournal of visualized experiments : JoVE2014
High throughput microinjections of sea urchin zygotes.
Article in Journal of visualized experiments : JoVE, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 25 citations in OpenAlex.
- Optimized protocols for generating half-sized embryos from separated first two blastomeres in green sea urchin andFrontiers in cell and developmental biology · 2025Article
- microRNA-1 regulates sea urchin skeletogenesis by directly targeting skeletogenic genes and modulating components of signaling pathways.Developmental biology · 2024Article
- Transcription of microRNAs is regulated by developmental signaling pathways and transcription factors.Frontiers in cell and developmental biology · 2024Article
- microRNA-124 directly suppresses Nodal and Notch to regulate mesodermal development.Developmental biology · 2023Article
- Large-scale F0 CRISPR screens in vivo using MIC-Drop.Nature protocols · 2023Review
- microRNA-124 regulates Notch and NeuroD1 to mediate transition states of neuronal development.Developmental neurobiology · 2023Article
- Article
- NeuroD1 localizes to the presumptive ganglia and gut of the sea urchin larvae.microPublication biology · 2022Article
- microRNA-31 regulates skeletogenesis by direct suppression of Eve and Wnt1.Developmental biology · 2021Article
- Rab35 regulates skeletogenesis and gastrulation by facilitating actin remodeling and vesicular trafficking.Cells & development · 2021Article
- Targeted Gene Disruption in Pacific Oyster Based on CRISPR/Cas9 Ribonucleoprotein Complexes.Marine biotechnology (New York, N.Y.) · 2019Article
- A Survey on Tubulin and Arginine Methyltransferase Families Sheds Light onInternational journal of molecular sciences · 2019Article
- Analysis of microRNA functions.Methods in cell biology · 2019Review
- Inhibition of microRNA suppression ofDevelopment (Cambridge, England) · 2018Article
- The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions.Journal of visualized experiments : JoVE · 2017Article
- microRNA-31 modulates skeletal patterning in the sea urchin embryo.Development (Cambridge, England) · 2015Article
- microRNAs regulate β-catenin of the Wnt signaling pathway in early sea urchin development.Developmental biology · 2015Article
- Expression of fluorescent proteins in Branchiostoma lanceolatum by mRNA injection into unfertilized oocytes.Journal of visualized experiments : JoVE · 2015Article
- The small GTPase Arf6 regulates sea urchin morphogenesis.Differentiation; research in biological diversityArticle
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Microinjection into cells and embryos is a common technique that is used to study a wide range of biological processes. In this method a small amount of treatment solution is loaded into a microinjection needle that is used to physically inject individual immobilized cells or embryos. Despite the need for initial training to perform this procedure for high-throughput delivery, microinjection offers maximum efficiency and reproducible delivery of a wide variety of treatment solutions (including complex mixtures of samples) into cells, eggs or embryos. Applications to microinjections include delivery of DNA constructs, mRNAs, recombinant proteins, gain of function, and loss of function reagents. Fluorescent or colorimetric dye is added to the injected solution to enable instant visualization of efficient delivery as well as a tool for reliable normalization of the amount of the delivered solution. The described method enables microinjection of 100-400 sea urchin zygotes within 10-15 min.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.