ArticleeLife2014
Single-molecule tracking of the transcription cycle by sub-second RNA detection.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
49 citing papers in PubMed, 79 citations in OpenAlex.
- Heterogeneous template-dependent transcription dynamics of T7 RNAP revealed by single-molecule imaging.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Tunable fluorogenic DNA probes drive fast and high-resolution single-molecule fluorescence imaging.Nucleic acids research · 2025Article
- Deciphering HIV-1 Transcription Initiation and Elongation from Single-Molecule Imaging Data.Research (Washington, D.C.) · 2025Article
- Imaging the Architecture of Granulomas Induced by Mycobacterium tuberculosis Infection with Single-molecule Fluorescence In Situ Hybridization.Journal of immunology (Baltimore, Md. : 1950) · 2024Article
- 5'UTR G-quadruplex structure enhances translation in size dependent manner.Nature communications · 2024Article
- A genetic circuit on a single DNA molecule as an autonomous dissipative nanodevice.Nature communications · 2024Article
- Bleaching-resistant, Near-continuous Single-molecule Fluorescence and FRET Based on Fluorogenic and Transient DNA Binding.Chemphyschem : a European journal of chemical physics and physical chemistry · 2023Article
- RT-IVT method allows multiplex real-time quantification of in vitro transcriptional mRNA production.Communications biology · 2023Article
- ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ.Nature methods · 2022Article
- A set of Saccharomyces cerevisiae integration vectors for fluorescent dye labeling of proteins.G3 (Bethesda, Md.) · 2022Article
- Viral detection and identification in 20 min by rapid single-particle fluorescence in-situ hybridization of viral RNA.Scientific reports · 2021Article
- How Single-Molecule Localization Microscopy Expanded Our Mechanistic Understanding of RNA Polymerase II Transcription.International journal of molecular sciences · 2021Review
- Article
- Dynamics of RNA polymerase II and elongation factor Spt4/5 recruitment during activator-dependent transcription.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cell-free biosensors for rapid detection of water contaminants.Nature biotechnology · 2020Article
- Real-time 3D single molecule tracking.Nature communications · 2020Article
- R-loop induced G-quadruplex in non-template promotes transcription by successive R-loop formation.Nature communications · 2020Article
- A combinatorial view of old and new RNA polymerase II modifications.Transcription · 2020Review
- Article
- AID-RNA polymerase II transcription-dependent deamination of IgV DNA.Nucleic acids research · 2019Article
Corrections and comments
- Commented on by
- Commented on by
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transcription is an inherently stochastic, noisy, and multi-step process, in which fluctuations at every step can cause variations in RNA synthesis, and affect physiology and differentiation decisions in otherwise identical cells. However, it has been an experimental challenge to directly link the stochastic events at the promoter to transcript production. Here we established a fast fluorescence in situ hybridization (fastFISH) method that takes advantage of intrinsically unstructured nucleic acid sequences to achieve exceptionally fast rates of specific hybridization (∼10e7 M(-1)s(-1)), and allows deterministic detection of single nascent transcripts. Using a prototypical RNA polymerase, we demonstrated the use of fastFISH to measure the kinetic rates of promoter escape, elongation, and termination in one assay at the single-molecule level, at sub-second temporal resolution. The principles of fastFISH design can be used to study stochasticity in gene regulation, to select targets for gene silencing, and to design nucleic acid nanostructures. DOI: http://dx.doi.org/10.7554/eLife.01775.001.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.