ArticleeLife2014
pH- and sodium-induced changes in a sodium/proton antiporter.
Article in eLife, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Architecture and rearrangements of a sperm-specific NaResearch square · 2023Article
- Crystal structure of the NaNature communications · 2022Article
- The Remaining Conundrum of the Role of the NaReviews in cardiovascular medicine · 2022Review
- Forty years in cryoEM of membrane proteins.Microscopy (Oxford, England) · 2022Article
- Expression of SSEA-4 and Oct-4 from somatic cells in primary mouse gastric cell culture induced by brief strong acid.Molecular and cellular biochemistry · 2021Article
- The change of intracellular zinc distribution after strong acid challenge.International journal of physiology, pathophysiology and pharmacology · 2021Article
- Structural and Functional Changes in the NaInternational journal of molecular sciences · 2019Review
- Functional Analysis of Conserved Transmembrane Charged Residues and a Yeast Specific Extracellular Loop of the Plasma Membrane NaScientific reports · 2019Article
- Broad phylogenetic analysis of cation/proton antiporters reveals transport determinants.Nature communications · 2018Article
- Critical Functions of Region 1-67 and Helix XIII in Retaining the Active Structure of NhaD Antiporter inFrontiers in microbiology · 2018Article
- Dissecting the proton transport pathway in electrogenic NaProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Simulating the Function of the MjNhaP1 Transporter.The journal of physical chemistry. B · 2016Article
- Expression and characterization of the SOS1 Arabidopsis salt tolerance protein.Molecular and cellular biochemistry · 2016Article
- Crystal structures reveal the molecular basis of ion translocation in sodium/proton antiporters.Nature structural & molecular biology · 2016Article
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- Repeated administration of inhibitors for ion pumps reduce markedly tumor growth in vivo.Medical archives (Sarajevo, Bosnia and Herzegovina) · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We examined substrate-induced conformational changes in MjNhaP1, an archaeal electroneutral Na(+)/H(+)-antiporter resembling the human antiporter NHE1, by electron crystallography of 2D crystals in a range of physiological pH and Na(+) conditions. In the absence of sodium, changes in pH had no major effect. By contrast, changes in Na(+) concentration caused a marked conformational change that was largely pH-independent. Crystallographically determined, apparent dissociation constants indicated ∼10-fold stronger Na(+) binding at pH 8 than at pH 4, consistent with substrate competition for a common ion-binding site. Projection difference maps indicated helix movements by about 2 Å in the 6-helix bundle region of MjNhaP1 that is thought to contain the ion translocation site. We propose that these movements convert the antiporter from the proton-bound, outward-open state to the Na(+)-bound, inward-open state. Oscillation between the two states would result in rapid Na(+)/H(+) antiport. DOI: http://dx.doi.org/10.7554/eLife.01412.001.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.