ArticleMolecular therapy. Nucleic acids2014
Two interconvertible folds modulate the activity of a DNA aptamer against transferrin receptor.
Article in Molecular therapy. Nucleic acids, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Aptamer-based technologies for extracellular vesicle analysis and engineering: from isolation to functional applications.Extracellular vesicles and circulating nucleic acids · 2026Review
- Bimodal brush-functionalized nanoparticles selective to receptor surface density.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Selection and identification of an RNA aptamer that specifically binds the HIV-1 capsid lattice and inhibits viral replication.Nucleic acids research · 2022Article
- Rationally designed DNA therapeutics can modulate human TH expression by controlling specific GQ formation in its promoter.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- A GATA3 Targeting Nucleic Acid Nanocapsule forACS nano · 2021Article
- Non-Invasive Delivery of Therapeutics into the Brain: The Potential of Aptamers for Targeted Delivery.Biomedicines · 2020Review
- Nanocarriers for Protein Delivery to the Cytosol: Assessing the Endosomal Escape of Poly(Lactide-co-Glycolide)-Poly(Ethylene Imine) Nanoparticles.Nanomaterials (Basel, Switzerland) · 2019Article
- Modular cell-internalizing aptamer nanostructure enables targeted delivery of large functional RNAs in cancer cell lines.Nature communications · 2018Article
- Toward the Selection of Cell Targeting Aptamers with Extended Biological Functionalities to Facilitate Endosomal Escape of Cargoes.Biomedicines · 2017Review
- A New Transferrin Receptor Aptamer Inhibits New World Hemorrhagic Fever Mammarenavirus Entry.Molecular therapy. Nucleic acids · 2016Article
- Improved Aptamers for the Diagnosis and Potential Treatment of HER2-Positive Cancer.Pharmaceuticals (Basel, Switzerland) · 2016Article
- Aptamer-Mediated Codelivery of Doxorubicin and NF-κB Decoy Enhances Chemosensitivity of Pancreatic Tumor Cells.Molecular therapy. Nucleic acids · 2015Article
- Therapeutic aptamers march on.Molecular therapy. Nucleic acids · 2014Article
- Aptamer technology for tracking cells' status & function.Molecular and cellular therapies · 2014Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Thanks to their ability to recognize biomolecular targets with high affinity and specificity, nucleic acid aptamers are increasingly investigated as diagnostic and therapeutic tools, particularly when their targets are cell-surface receptors. Here, we investigate the relationship between the folding of an anti-mouse transferrin receptor DNA aptamer and its interaction with the transferrin receptor both in vitro and in living cells. We identified and purified two aptamer conformers by means of chromatographic techniques. Fluorescence-anisotropy measurements showed that only one fold is able to bind mouse transferrin receptor. Besides displaying enhanced endocytosis in living mouse fibroblasts, the purified active fold is internalized also in human pancreatic cancer cells. Starting from these observations, we rationally designed variations of the parent sequence aimed at stabilizing the active fold, and consequently increase aptamer activity. A truncated version and full-length mutants with higher affinity than the parent sequence are shown.Molecular Therapy-Nucleic Acids (2014) 3, e144; doi:10.1038/mtna.2013.71; published online 28 January 2014.
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