Evidence map›Paper›PMID 24472845›Full record

ArticleMolecular and cellular neurosciences2014

Monocytes are essential for the neuroprotective effect of human cord blood cells following middle cerebral artery occlusion in rat.

T A Womble, S Green, M Shahaduzzaman, J Grieco, P R Sanberg, K R Pennypacker, A E Willing

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular and cellular neurosciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.

0numbers the graph read from it
0cells of the map it votes in
29citing papers in PubMed
4.6field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

29 citing papers in PubMed, 47 citations in OpenAlex.

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  13. Endogenous Protection from Ischemic Brain Injury by Preconditioned Monocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

T A WombleCenter for Excellence in Aging and Brain Repair,University of South Florida, Tampa, FL 33612, United States; Department of Neurosurgery and Brain Repair, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, United States.
S GreenCenter for Excellence in Aging and Brain Repair,University of South Florida, Tampa, FL 33612, United States; Department of Neurosurgery and Brain Repair, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, United States.
M ShahaduzzamanCenter for Excellence in Aging and Brain Repair,University of South Florida, Tampa, FL 33612, United States; Department of Neurosurgery and Brain Repair, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, United States.
J GriecoCenter for Excellence in Aging and Brain Repair,University of South Florida, Tampa, FL 33612, United States.
P R SanbergCenter for Excellence in Aging and Brain Repair,University of South Florida, Tampa, FL 33612, United States; Department of Neurosurgery and Brain Repair, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, United States.
K R PennypackerCenter for Excellence in Aging and Brain Repair,University of South Florida, Tampa, FL 33612, United States; Department of Molecular Pharmacology and Physiology, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, United States.
A E WillingCenter for Excellence in Aging and Brain Repair,University of South Florida, Tampa, FL 33612, United States; Department of Neurosurgery and Brain Repair, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, United States; Department of Molecular Pharmacology and Physiology, University of South Florida, 12901 Bruce B. Downs Blvd, Tampa, FL 33612, United States. Electronic address: awilling@health.usf.edu.
University of South Florida · US

Funding

Cord Blood is Neuroprotective in a Rat Model of StrokeR01NS052839 · NINDS · UNIVERSITY OF SOUTH FLORIDA · PI WILLING, ALISON E · 2006 to 2010
$1.8M
NINDS NIH HHS R01 NS052839NINDS NIH HHS R01NS52839
6 · The paper itself

Abstract

Systemic administration of human umbilical cord blood (HUCB) mononuclear cells (MNC) following middle cerebral artery occlusion (MCAO) in the rat reduces infarct size and, more importantly, restores motor function. The HUCB cell preparation is composed of immature T-cells, B-cells, monocytes and stem cells. In this study we examined whether the beneficial effects of HUCB injection were attributable to one of these cell types. Male Sprague Dawley rats underwent permanent MCAO followed 48 h later by intravenous administration of HUCB MNC preparations depleted of either CD14(+) monocytes, CD133(+) stem cells, CD2(+) T-cells or CD19(+) B cells. Motor function was measured prior to MCAO and 30 days post-stroke. When CD14(+) monocytes were depleted from the HUCB MNC, activity and motor asymmetry were similar to the MCAO only treated animals. Monocyte depletion prevented HUCB cell treatment from reducing infarct size while monocyte enrichment was sufficient to reduce infarct size. Administration of monocyte-depleted HUCB cells did not suppress Iba1 labeling of microglia in the infarcted area relative to treatment with the whole HUCB preparation. These data demonstrate that the HUCB monocytes provide the majority of the efficacy in reducing infarct volume and promoting functional recovery.

Indexed as

AC133 AntigenAnimalsAntigens, CDAntigens, CD19B-LymphocytesCD2 AntigensFetal BloodGlycoproteinsHematopoietic Stem CellsHumansInfarction, Middle Cerebral ArteryLipopolysaccharide ReceptorsMaleMonocytesPeptidesRatsAC133 AntigenAntigens, CDAntigens, CD19CD2 AntigensGlycoproteinsLipopolysaccharide ReceptorsPeptidesPROM1 protein, humanProm1 protein, ratBehaviorCerebral ischemiaCord bloodInfarctMicrogliaMonocytes

Identifiers

PMID24472845
PMCPMC4008706
OpenAlexW1991269909

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.