ArticleParasites & vectors2014
Anopheles sinensis mosquito insecticide resistance: comparison of three mosquito sample collection and preparation methods and mosquito age in resistance measurements.
Article in Parasites & vectors, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 31 citations in OpenAlex.
- Development of a multiplex SNaPshot assay for simultaneous detection of multiple insecticide-resistance mutations in Anopheles sinensis.Parasites & vectors · 2026Article
- Impact of the V410L kdr mutation and co-occurring genotypes at kdr sites 1016 and 1534 in the VGSC on the probability of survival of the mosquito Aedes aegypti (L.) to Permanone in Harris County, TX, USA.PLoS neglected tropical diseases · 2023Article
- Potential of Essential Oil-Based Anticholinesterase Insecticides againstMolecules (Basel, Switzerland) · 2022Review
- Community structure and insecticide resistance of malaria vectors in northern-central Myanmar.Parasites & vectors · 2022Article
- NEVBD Pesticide Resistance Monitoring Network: Establishing a Centralized Network to Increase Regional Capacity for Pesticide Resistance Detection and Monitoring.Journal of medical entomology · 2021Article
- Development of miRNA-Based Approaches to Explore the Interruption of Mosquito-Borne Disease Transmission.Frontiers in cellular and infection microbiology · 2021Review
- Article
- Characterizing the molecular and metabolic mechanisms of insecticide resistance in Anopheles gambiae in Faranah, Guinea.Malaria journal · 2019Article
- The influence of age on insecticide susceptibility of Anopheles arabiensis during dry and rainy seasons in rice irrigation schemes of Northern Tanzania.Malaria journal · 2017Article
- Evaluation of Larvicidal Activity of Essential Oil from Leaves ofJournal of arthropod-borne diseases · 2017Article
- Evaluation of Insecticides Susceptibility and Malaria Vector Potential of Anopheles annularis s.l. and Anopheles vagus in Assam, India.PloS one · 2016Article
- Computational identification of novel microRNAs and their targets in the malarial vector, Anopheles stephensi.Systems and synthetic biology · 2015Article
- Knockdown resistance of Anopheles sinensis in Henan province, China.Malaria journal · 2015Article
- Modelling the impact of declining insecticide resistance with mosquito age on malaria transmission.MalariaWorld journal · 2015Article
- Genomic Analysis of Detoxification Supergene Families in the Mosquito Anopheles sinensis.PloS one · 2015Article
- Identification of differentially expressed microRNAs in Culex pipiens and their potential roles in pyrethroid resistance.Insect biochemistry and molecular biology · 2014Article
- Development and application of an AllGlo probe-based qPCR assay for detecting knockdown resistance (kdr) mutations in Anopheles sinensis.Malaria journal · 2014Article
- Transcriptome profiling of pyrethroid resistant and susceptible mosquitoes in the malaria vector, Anopheles sinensis.BMC genomics · 2014Article
- Multiple resistances and complex mechanisms of Anopheles sinensis mosquito: a major obstacle to mosquito-borne diseases control and elimination in China.PLoS neglected tropical diseases · 2014Article
- Insecticide resistance of Anopheles sinensis and An. vagus in Hainan Island, a malaria-endemic area of China.Parasites & vectors · 2014Article
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundInsecticide resistance monitoring in malaria mosquitoes is essential for guiding the rational use of insecticides in vector control programs. Resistance bioassay is the first step for insecticide monitoring and it lays an important foundation for molecular examination of resistance mechanisms. In the literature, various mosquito sample collection and preparation methods have been used, but how mosquito sample collection and preparation methods affect insecticide susceptibility bioassay results is largely unknown. The objectives of this study were to determine whether mosquito sample collection and preparation methods affected bioassay results, which may cause incorrect classification of mosquito resistance status.
methodsThe study was conducted in Anopheles sinensis mosquitoes in two study sites in central China. Three mosquito sample collection and preparation methods were compared for insecticide susceptibility, kdr frequencies and metabolic enzyme activities: 1) adult mosquitoes collected from the field; 2) F1 adults from field collected, blood-fed mosquitoes; and 3) adult mosquitoes reared from field collected larvae.
resultsMosquito sample collection and preparation methods significantly affected mortality rates in the standard WHO tube resistance bioassay. Mortality rate of field-collected female adults was 10-15% higher than in mosquitoes reared from field-collected larvae and F1 adults from field collected blood-fed females. This pattern was consistent in mosquitoes from the two study sites. High kdr mutation frequency (85-95%) with L1014F allele as the predominant mutation was found in our study populations. Field-collected female adults consistently exhibited the highest monooxygenase and GST activities. The higher mortality rate observed in the field-collected female mosquitoes may have been caused by a mixture of mosquitoes of different ages, as older mosquitoes were more susceptible to deltamethrin than younger mosquitoes.
conclusionsFemale adults reared from field-collected larvae in resistance bioassays are recommended to minimize the effect of confounding factors such as mosquito age and blood feeding status so that more reliable and reproducible mortality may be obtained.
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