ArticleAmerican journal of respiratory cell and molecular biology2014
Tissue optical clearing, three-dimensional imaging, and computer morphometry in whole mouse lungs and human airways.
Article in American journal of respiratory cell and molecular biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 63 citations in OpenAlex.
- The Role of the Nervous System in Lung Disease.Current neurology and neuroscience reports · 2026Review
- Multidimensional lung imaging: integrated preclinical platforms enabling the identification of translational biomarkers for pulmonary research.Respiratory research · 2026Review
- Article
- Maternal high-fat diet programs offspring airway hyperinnervation and hyperresponsiveness.JCI insight · 2025Article
- Impact of CD11cFrontiers in fungal biology · 2025Article
- Review
- Neurobiology of Coughing in Children.Journal of clinical medicine · 2023Review
- Maternal high-fat diet increases airway sensory innervation and reflex bronchoconstriction in adult offspring.American journal of physiology. Lung cellular and molecular physiology · 2023Article
- Optical clearing and testing of lung tissue using inhalation aerosols: prospects for monitoring the action of viral infections.Biophysical reviews · 2022Review
- Optical tissue clearing associated with 3D imaging: application in preclinical and clinical studies.Histochemistry and cell biology · 2022Review
- Multicolor labeling of airway neurons and analysis of parasympathetic heterogeneity.Scientific reports · 2022Article
- Identification of lung innervating sensory neurons and their target specificity.American journal of physiology. Lung cellular and molecular physiology · 2022Article
- Mini review: Neural mechanisms underlying airway hyperresponsiveness.Neuroscience letters · 2021Review
- Physical and chemical mechanisms of tissue optical clearing.iScience · 2021Review
- Airway Sensory Nerve Density Is Increased in Chronic Cough.American journal of respiratory and critical care medicine · 2021Article
- New Look at RSV Infection: Tissue Clearing and 3D Imaging of the Entire Mouse Lung at Cellular Resolution.Viruses · 2021Article
- Molecular identity, anatomy, gene expression and function of neural crest vs. placode-derived nociceptors in the lower airways.Neuroscience letters · 2021Review
- Airway Sensory Nerve Plasticity in Asthma and Chronic Cough.Frontiers in physiology · 2021Review
- Tissue Optical Clearing for Biomedical Imaging: From In Vitro to In Vivo.Advances in experimental medicine and biology · 2021Article
- Biomedical Applications of Tissue Clearing and Three-Dimensional Imaging in Health and Disease.iScience · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In whole adult mouse lung, full identification of airway nerves (or other cellular/subcellular objects) has not been possible due to patchy distribution and micron-scale size. Here we describe a method using tissue clearing to acquire the first complete image of three-dimensional (3D) innervation in the lung. We then created a method to pair analysis of nerve (or any other colabeled epitope) images with identification of 3D tissue compartments and airway morphometry by using fluorescent casting and morphometry software (which we designed and are making available as open-source). We then tested our method to quantify a sparse heterogeneous nerve population by examining visceral pleural nerves. Finally, we demonstrate the utility of our method in human tissue to image full thickness innervation in irregular 3D tissue compartments and to quantify sparse objects (intrinsic airway ganglia). Overall, this method can uniquely pair the advantages of whole tissue imaging and cellular/subcellular fluorescence microscopy.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.