ArticleInfection and immunity2014
Elucidating the genetic basis of crystalline biofilm formation in Proteus mirabilis.
Article in Infection and immunity, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 62 citations in OpenAlex.
- Surface modification of urinary catheters with antibiofilm nanocoating: curcumin modified silver nanoparticles against Proteus mirabilis.Journal of materials science. Materials in medicine · 2026Article
- Effect of metabolites on the survival and biofilm formation of Pantoea piersonii (Basionym: Kalamiella piersonii) in synthetic urine media.BMC microbiology · 2026Article
- Ultrasonic irradiation in the synthesis of nanohydroxyapatite: a chemically friendly technique for improving hemocompatibility and antibiofilm applications.Beilstein journal of nanotechnology · 2026Article
- Genomic Characterization of a Class 2/1 Hybrid Integron-Driven Multidrug-ResistantVeterinary sciences · 2025Article
- Decoding Proteus mirabilis biofilms: expression of selected virulence genes and association with antibiotic resistance.BMC microbiology · 2025Article
- Evaluation of Bcr/cflA Targeted Efflux Inhibitory Potential of 2-Hydroxy-4-Methoxybenzaldehyde AgainstIndian journal of microbiology · 2025Article
- Decoding the regulatory networks ofFrontiers in cellular and infection microbiology · 2025Article
- Green-Synthesized Silver Nanoparticles in the Prevention of Multidrug-Resistant Proteus mirabilis Infection and Incrustation of Urinary Catheters BioAgNPs Against P. mirabilis Infection.Current microbiology · 2024Article
- Targeting bacterial biofilm-related genes with nanoparticle-based strategies.Frontiers in microbiology · 2024Review
- Efflux pumps as potential targets for biofilm inhibition.Frontiers in microbiology · 2024Review
- What's old is new again: Insights into diabetic foot microbiome.World journal of diabetes · 2023Review
- Infection responsive coatings to reduce biofilm formation and encrustation of urinary catheters.Journal of applied microbiology · 2023Article
- Biofilm Lifestyle in Recurrent Urinary Tract Infections.Life (Basel, Switzerland) · 2023Review
- The molybdate-binding protein ModA is required forFrontiers in microbiology · 2023Article
- Lipopolysaccharide structure modulates cationic biocide susceptibility and crystalline biofilm formation inFrontiers in microbiology · 2023Article
- Genetic analysis of resistance and virulence characteristics of clinical multidrug-resistantFrontiers in cellular and infection microbiology · 2023Article
- A biogeographic 16S rRNA survey of bacterial communities of ureolytic biomineralization from California public restrooms.PloS one · 2022Article
- Virulence genes and antimicrobial resistance pattern in Proteus mirabilis strains isolated from patients attended with urinary infections to Tertiary Hospitals, in Iran.African health sciences · 2021Article
- Efficacy of three lytic bacteriophages for eradicating biofilms of multidrug-resistant Proteus mirabilis.Archives of virology · 2021Article
- Relevance of iron metabolic genes in biofilm and infection in uropathogenicCurrent research in microbial sciences · 2021Article
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 1 country.
Funding
Abstract
Proteus mirabilis forms extensive crystalline biofilms on urethral catheters that occlude urine flow and frequently complicate the management of long-term-catheterized patients. Here, using random transposon mutagenesis in conjunction with in vitro models of the catheterized urinary tract, we elucidate the mechanisms underpinning the formation of crystalline biofilms by P. mirabilis. Mutants identified as defective in blockage of urethral catheters had disruptions in genes involved in nitrogen metabolism and efflux systems but were unaffected in general growth, survival in bladder model systems, or the ability to elevate urinary pH. Imaging of biofilms directly on catheter surfaces, along with quantification of levels of encrustation and biomass, confirmed that the mutants were attenuated specifically in the ability to form crystalline biofilms compared with that of the wild type. However, the biofilm-deficient phenotype of these mutants was not due to deficiencies in attachment to catheter biomaterials, and defects in later stages of biofilm development were indicated. For one blocking-deficient mutant, the disrupted gene (encoding a putative multidrug efflux pump) was also found to be associated with susceptibility to fosfomycin, and loss of this system or general inhibition of efflux pumps increased sensitivity to this antibiotic. Furthermore, homologues of this system were found to be widely distributed among other common pathogens of the catheterized urinary tract. Overall, our findings provide fundamental new insight into crystalline biofilm formation by P. mirabilis, including the link between biofilm formation and antibiotic resistance in this organism, and indicate a potential role for efflux pump inhibitors in the treatment or prevention of P. mirabilis crystalline biofilms.
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