ArticlePhilosophical transactions. Series A, Mathematical, physical, and engineering sciences2014
Scanning electron microscope studies of human metaphase chromosomes.
Article in Philosophical transactions. Series A, Mathematical, physical, and engineering sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- MetaChrome: an open-source, user-friendly tool for automated metaphase chromosome analysis.Methods (San Diego, Calif.) · 2026Article
- Uncovering mitotic ultrastructure in the native hair follicle using volume electron microscopy.Journal of cell science · 2026Article
- MetaChrome: An Open-Source, User-Friendly Tool for Automated Metaphase Chromosome Analysis.bioRxiv : the preprint server for biology · 2025Article
- Large-field-of-view scanning electron microscopy of the paraventricular nucleus of the hypothalamus during diet-induced obesity.Journal of neurophysiology · 2023Article
- Cryo-nanoscale chromosome imaging-future prospects.Biophysical reviews · 2020Review
- Use of 3D imaging for providing insights into high-order structure of mitotic chromosomes.Chromosoma · 2019Review
- The use of DAPI fluorescence lifetime imaging for investigating chromatin condensation in human chromosomes.Scientific reports · 2016Article
- Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.Scientific reports · 2016Article
- Structural organization of very small chromosomes: study on a single-celled evolutionary distant eukaryote Giardia intestinalis.Chromosoma · 2015Article
- Karyotyping human chromosomes by optical and X-ray ptychography methods.Biophysical journal · 2015Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Scanning electron microscopy (SEM) is used to evaluate potential chromosome preparations and staining methods for application in high-resolution three-dimensional X-ray imaging. Our starting point is optical fluorescence microscopy, the standard method for chromosomes, which only gives structural detail at the 200 nm scale. In principle, with suitable sample preparation protocols, including contrast enhancing staining, the surface structure of the chromosomes can be viewed at the 1 nm level by SEM. Here, we evaluate a heavy metal nucleic-acid-specific stain, which gives strong contrast in the backscattered electron signal. This study uses SEM to examine chromosomes prepared in different ways to establish a sample preparation protocol for X-rays. Secondary electron and backscattered electron signals are compared to evaluate the effectiveness of platinum-based stains used to enhance the contrast.
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