Evidence map›Paper›PMID 24469315›Full record

ArticleInternational journal of molecular sciences2014

Protein a detection based on quantum dots-antibody bioprobe using fluorescence coupled capillary electrophoresis.

Lin Qiu, Yanhua Bi, Cheli Wang, Jingyan Li, Peilin Guo, Jinchen Li, Weijiang He, Jianhao Wang, Pengju Jiang

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Lin QiuState Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. linqiupjj@gmail.com.
Yanhua BiShandong Academy of Medical Sciences, Jinan 250062, China. byh168@163.com.
Cheli WangSchool of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. clwang@cczu.edu.cn.
Jingyan LiSchool of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. jingyan.leee@gmail.com.
Peilin GuoSchool of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. 351533126@163.com.
Jinchen LiSchool of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. jingyan.leee@gmail.com.
Weijiang HeState Key Laboratory of Coordination Chemistry, Coordination Chemistry Institute, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China. heweij69@nju.edu.cn.
Jianhao WangSchool of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. minuswan@gmail.com.
Pengju JiangSchool of Pharmaceutical Engineering and Life Science, Changzhou University, Changzhou 213164, China. pengju.jiang@gmail.com.
Changzhou University · CNNanjing University · CNShandong First Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this report, fluorescence detection coupled capillary electrophoresis (CE-FL) was used to detect Protein A. Antibody was first labeled with Cy5 and then mixed with quantum dots (QDs) to form QDs-antibody bioprobe. Further, we observed fluorescence resonance energy transfer (FRET) from QDs donor to Cy5 acceptor. The bioprobe was formed and brought QDs and Cy5 close enough to allow FRET to occur. After adding protein A, the FRET system was broken and caused the FRET signal to decrease. Thus, a new method for the determination of protein A was proposed based on the FRET signal changes. This study provides a new trail of thought for the detection of protein.

Indexed as

Biosensing TechniquesElectrophoresis, CapillaryFluorescence Resonance Energy TransferAntibodiesCarbocyaninesImmunoglobulin GQuantum DotsStaphylococcal Protein AAntibodiesCarbocyaninescyanine dye 5Immunoglobulin GStaphylococcal Protein A

Identifiers

PMID24469315
PMCPMC3958821
OpenAlexW1969040099

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.