ArticleInternational journal of obesity (2005)2014
Overexpression of gastric leptin precedes adipocyte leptin during high-fat diet and is linked to 5HT-containing enterochromaffin cells.
Article in International journal of obesity (2005), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 33 citations in OpenAlex.
- Adipocytokine Protein Expression from Visceral Fat Differs Significantly Based on Diet, Sex, and Age in C3H/HeJ Mice Fed Long-Term, High-Fat Diets, ± Ammonium-Hydroxide-Supplemented Dietary Protein.Current issues in molecular biology · 2025Article
- Leptin receptor reactivation restores brain function in early-life Lepr-deficient mice.Brain : a journal of neurology · 2024Article
- A Focus on Enterochromaffin Cells among the Enteroendocrine Cells: Localization, Morphology, and Role.International journal of molecular sciences · 2022Review
- Metabolic Disturbances in Rat Sublines with Constitutionally Altered Serotonin Homeostasis.International journal of molecular sciences · 2021Article
- Serotonin-Its Synthesis and Roles in the Healthy and the Critically Ill.International journal of molecular sciences · 2021Review
- The Leptin System and Diet: A Mini Review of the Current Evidence.Frontiers in endocrinology · 2021Review
- Leptin-mediated differential regulation of microsomal triglyceride transfer protein in the intestine and liver affects plasma lipids.The Journal of biological chemistry · 2020Article
- Sugar Responses of Human Enterochromaffin Cells Depend on Gut Region, Sex, and Body Mass.Nutrients · 2019Article
- Augmented capacity for peripheral serotonin release in human obesity.International journal of obesity (2005) · 2018Article
- The Regulation of Peripheral Metabolism by Gut-Derived Hormones.Frontiers in endocrinology · 2018Review
- Time course metabolome of Roux-en-Y gastric bypass confirms correlation between leptin, body weight and the microbiome.PloS one · 2018Article
- The gut sensor as regulator of body weight.Endocrine · 2015Review
- Alzheimer-associated Aβ oligomers impact the central nervous system to induce peripheral metabolic deregulation.EMBO molecular medicine · 2015Article
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesIn obesity, while hyperleptinemia highly correlates with excess fat mass, the status of gastric leptin remains unknown. Here, we investigated the expression of leptin in stomach biopsies of obese humans and analyzed the temporal changes of gastric leptin expression in response to diet-induced obesity and its impact on 5-hydroxytryptamine (5HT)-producing cells.
methodsEnterochromaffin (EC) cells and expression of leptin, PAX4 (critical factor for EC specification), tryptophane hydroxylase-1 (TPH1, the peripheral rate-limiting enzyme for 5HT) and 5HT were examined by immunofluorescence, quantitative real-time PCR, radioimmunoassay, respectively, in stomach and duodenum biopsies from 19 obese and 14 normo-weighed individuals, and in mucosa scrapings from C57Bl6/J diet-induced obese mice, leptin-deficient ob/ob mice and intestine-specific leptin receptor isoform B-deficient mice.
resultsGastric mucosa of obese subjects displays an increased expression of leptin (LEP mRNA by fivefold and protein by twofold, P<0.01), TPH1 ((1.75-2.73, 95% confidence interval (CI)) vs (0.38-0.67, 95% CI); P<0.01) and PAX4 ((1.33-2.11, 95%CI) vs (0.62-0.81, 95% CI); P<0.01) as compared with normo-weighed individuals. In diet-induced obese mice, the overexpressions of gastric leptin, antral Pax4, Tph1 and increased EC cell number occurred before the onset of obesity and hyperleptinemia (reflect of adipocyte leptin production). In addition, leptin deficiency was associated with reduced Pax4 mRNA, whereas oral leptin treatment enhanced both Tph1 and Pax4 mRNA. Finally, mice with an intestine-specific deletion of leptin signaling exhibit significant decrease in duodenal mucosa 5HT content.
conclusionsThese data demonstrate that gastric leptin is upregulated in obese individuals. RESULTS from high-fat diet mice showed that overexpression of gastric leptin that is linked to gut '5HT pathway' occurred before the onset of obesity and expansion of fat mass. This may be relevant in the pathophysiology of obesity.
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