Evidence map›Paper›PMID 24468583›Full record

ArticleActa biomaterialia2014

Enhanced trophic factor secretion by mesenchymal stem/stromal cells with Glycine-Histidine-Lysine (GHK)-modified alginate hydrogels.

Soumia Jose, Marissa L Hughbanks, Bernard Y K Binder, Ganesh C Ingavle, J Kent Leach

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta biomaterialia, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.

0numbers the graph read from it
0cells of the map it votes in
38citing papers in PubMed
4.3field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

38 citing papers in PubMed, 74 citations in OpenAlex.

  1. Article
  2. Edible Sulfonated Soy Protein Microcarriers for Cultivated Meat Cell Expansion.Journal of biomedical materials research. Part A · 2026
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  13. Effect of Pore Size on Cell Behavior Using Melt Electrowritten Scaffolds.Frontiers in bioengineering and biotechnology · 2021
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Soumia JoseDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, USA.
Marissa L HughbanksDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, USA.
Bernard Y K BinderDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, USA.
Ganesh C IngavleDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, USA.
J Kent LeachDepartment of Biomedical Engineering, University of California, Davis, Davis, CA, USA; Department of Orthopaedic Surgery, University of California, Davis, School of Medicine, Sacramento, CA, USA. Electronic address: jkleach@ucdavis.edu.
University of California, Davis · US

Funding

Phospholipids for enhancing cell-based neovascularizationR21AG036963 · NIA · UNIVERSITY OF CALIFORNIA AT DAVIS · PI LEACH, J. KENT · 2010 to 2011
$339k
NIA NIH HHS R21 AG036963NIA NIH HHS R21AG036963
6 · The paper itself

Abstract

Recombinant proteins and cytokines are under broad preclinical and clinical investigation to promote angiogenesis, but their success is limited by ineffective delivery, lack of long-term stability and excessive cost. Mesenchymal stem/stromal cells (MSC) secrete bioactive trophic factors, and thus, may provide an effective alternative to address these challenges. Glycine-Histidine-Lysine (GHK) is a peptide fragment of osteonectin, a matricellular protein with reported proangiogenic potential. We examined the capacity of GHK to up-regulate secretion of proangiogenic factors from human MSC in culture and when covalently coupled to alginate hydrogels. GHK had no apparent cytotoxic effects on MSC in culture over a wide range of concentrations. We detected a dose-dependent increase in vascular endothelial growth factor (VEGF) concentration in media conditioned by GHK-treated MSC, which increased endothelial cell proliferation, migration and tubule formation. We covalently coupled GHK to alginate using carbodiimide chemistry, and human MSC were entrapped in alginate hydrogels to assess VEGF secretion. Similar to monolayer culture, MSC responded to GHK-modified gels by secreting increased concentrations of VEGF and basic fibroblast growth factor compared to unmodified gels. The pre-treatment of MSC with antibodies to α6 and β1 integrins prior to entrapment in GHK-modified gels abrogated VEGF secretion, suggesting that the proangiogenic response of MSC was integrin-mediated. These data demonstrate that the proangiogenic potential of MSC can be significantly increased by the presentation of GHK with a biodegradable carrier, therefore increasing their clinical potential when used for tissue repair.

Indexed as

AlginatesCell AdhesionCell DeathCell MovementCell SurvivalColony-Forming Units AssayCulture Media, ConditionedEndothelial CellsGlucuronic AcidHexuronic AcidsHumansHydrogelsIntegrinsMesenchymal Stem CellsMitogensOligopeptidesAlginatesCulture Media, ConditionedGlucuronic AcidHexuronic AcidsHydrogelsIntegrinsMitogensOligopeptidesVascular Endothelial Growth Factor AAlginateAngiogenesisGHKHydrogelVascular endothelial growth factor

Identifiers

PMID24468583
PMCPMC3976757
OpenAlexW2061460113

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.