ArticleBMC biology2014
Uncovering by atomic force microscopy of an original circular structure at the yeast cell surface in response to heat shock.
Article in BMC biology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
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- Thermotolerant yeasts promoting climate-resilient bioproduction.FEMS yeast research · 2025Review
- Yeast Strains as Probiotic and Postbiotic Agents for the Agglutination of Enteric Pathogens: A Preventive Approach.Pathogens (Basel, Switzerland) · 2025Article
- Combined transcriptomics and metabolomics analyses reveal the molecular mechanism of heat tolerance inFrontiers in microbiology · 2025Article
- Single-cell patterning and characterisation of antibiotic persistent bacteria using bio-sCAPA.Lab on a chip · 2023Article
- An Evolved Strain of the Oleaginous YeastJournal of fungi (Basel, Switzerland) · 2023Article
- Quantifying the Mechanical Properties of Yeast Candida albicans Using Atomic Force Microscopy-based Force Spectroscopy.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Ceragenin CSA-44 as a Means to Control the Formation of the Biofilm on the Surface of Tooth and Composite Fillings.Pathogens (Basel, Switzerland) · 2022Article
- Crosstalk between Yeast Cell Plasma Membrane Ergosterol Content and Cell Wall Stiffness under Acetic Acid Stress Involving Pdr18.Journal of fungi (Basel, Switzerland) · 2022Article
- The cell wall and the response and tolerance to stresses of biotechnological relevance in yeasts.Frontiers in microbiology · 2022Review
- Yeast adaptive response to acetic acid stress involves structural alterations and increased stiffness of the cell wall.Scientific reports · 2021Article
- Cell Sorting Using Electrokinetic Deterministic Lateral Displacement.Micromachines · 2020Article
- Using Atomic Force Microscopy To Illuminate the Biophysical Properties of Microbes.ACS applied bio materials · 2020Article
- Microbial adhesion and ultrastructure from the single-molecule to the single-cell levels by Atomic Force Microscopy.Cell surface (Amsterdam, Netherlands) · 2019Review
- AFM dendritips functionalized with molecular probes specific to cell wall polysaccharides as a tool to investigate cell surface structure and organization.Cell surface (Amsterdam, Netherlands) · 2019Article
- Insoluble solids at high concentrations repress yeast's response against stress and increase intracellular ROS levels.Scientific reports · 2019Article
- Integrins in disguise - mechanosensors inMicrobial cell (Graz, Austria) · 2019Review
- Genomic Insights Into Sugar Adaptation in an Extremophile YeastFrontiers in microbiology · 2019Article
- Adaption to stress via Pbs2 during Metarhizium rileyi conidia and microsclerotia development.World journal of microbiology & biotechnology · 2018Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAtomic Force Microscopy (AFM) is a polyvalent tool that allows biological and mechanical studies of full living microorganisms, and therefore the comprehension of molecular mechanisms at the nanoscale level. By combining AFM with genetical and biochemical methods, we explored the biophysical response of the yeast Saccharomyces cerevisiae to a temperature stress from 30°C to 42°C during 1 h.
resultsWe report for the first time the formation of an unprecedented circular structure at the cell surface that takes its origin at a single punctuate source and propagates in a concentric manner to reach a diameter of 2-3 μm at least, thus significantly greater than a bud scar. Concomitantly, the cell wall stiffness determined by the Young's Modulus of heat stressed cells increased two fold with a concurrent increase of chitin. This heat-induced circular structure was not found either in wsc1Δ or bck1Δ mutants that are defective in the CWI signaling pathway, nor in chs1Δ, chs3Δ and bni1Δ mutant cells, reported to be deficient in the proper budding process. It was also abolished in the presence of latrunculin A, a toxin known to destabilize actin cytoskeleton.
conclusionsOur results suggest that this singular morphological event occurring at the cell surface is due to a dysfunction in the budding machinery caused by the heat shock and that this phenomenon is under the control of the CWI pathway.
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