ArticleMalaria journal2014
Field evaluation of the photo-induced electron transfer fluorogenic primers (PET) real-time PCR for the detection of Plasmodium falciparum in Tanzania.
Article in Malaria journal, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Prevalence ofThe American journal of tropical medicine and hygiene · 2020Trial
- Asymptomatic Malaria Reservoirs in Honduras: A Challenge for Elimination.Pathogens (Basel, Switzerland) · 2024Article
- PET-PCR reveals low parasitaemia and submicroscopic malarial infections in Honduran Moskitia.Malaria journal · 2023Article
- Detecting asymptomatic carriage of Plasmodium falciparum in southern Ghana: utility of molecular and serological diagnostic tools.Malaria journal · 2022Article
- Article
- Field validation of a magneto-optical detection device (Gazelle) for portable point-of-care Plasmodium vivax diagnosis.PloS one · 2021Article
- Diagnostic Methods for Non-Falciparum Malaria.Frontiers in cellular and infection microbiology · 2021Review
- Template copy number and the sensitivity of quantitative PCR for Plasmodium falciparum in asymptomatic individuals.Malaria journal · 2020Article
- Targeted deep amplicon sequencing of kelch 13 and cytochrome b in Plasmodium falciparum isolates from an endemic African country using the Malaria Resistance Surveillance (MaRS) protocol.Parasites & vectors · 2020Article
- Next-Generation Sequencing and Bioinformatics Protocol for Malaria Drug Resistance Marker Surveillance.Antimicrobial agents and chemotherapy · 2018Article
- Emergence and Spread ofAntimicrobial agents and chemotherapy · 2018Article
- A sensitive species-specific reverse transcription real-time PCR method for detection ofParasite epidemiology and control · 2017Article
- Molecular Epidemiology of Plasmodium falciparum kelch13 Mutations in Senegal Determined by Using Targeted Amplicon Deep Sequencing.Antimicrobial agents and chemotherapy · 2017Observational
- Molecular diagnosis of Plasmodium ovale by photo-induced electron transfer fluorogenic primers: PET-PCR.PloS one · 2017Article
- Evaluation of the Illumigene Malaria LAMP: A Robust Molecular Diagnostic Tool for Malaria Parasites.Scientific reports · 2016Article
- Malaria elimination in Haiti by the year 2020: an achievable goal?Malaria journal · 2015Article
- Ultra-sensitive detection of Plasmodium falciparum by amplification of multi-copy subtelomeric targets.PLoS medicine · 2015Article
- Quality assurance of malaria rapid diagnostic tests used for routine patient care in rural Tanzania: microscopy versus real-time polymerase chain reaction.Malaria journal · 2015Article
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Abstract
backgroundAccurate diagnosis of malaria infections remains challenging, especially in the identification of submicroscopic infections. New molecular diagnostic tools that are inexpensive, sensitive enough to detect low-level infections and suitable in laboratory settings of resource-limited countries are required for malaria control and elimination programmes. Here the diagnostic potential of a recently developed photo-induced electron transfer fluorogenic primer (PET) real-time polymerase chain reaction (PCR) called PET-PCR was investigated. This study aimed to (i) evaluate the use of this assay as a method for the detection of both Plasmodium falciparum and other Plasmodium species infections in a developing country's diagnostic laboratory; and, (ii) determine the assay's sensitivity and specificity compared to a nested 18S rRNA PCR.
methodsSamples used in this study were obtained from a previous study conducted in the region of Iringa, Tanzania. A total of 303 samples from eight health facilities in Tanzania were utilized for this evaluation. All samples were screened using the multiplex PET-PCR assay designed to detect Plasmodium genus and P. falciparum initially in laboratory in Tanzania and then repeated at a reference laboratory at the CDC in the USA. Microscopy data was available for all the 303 samples. A subset of the samples were tested in a blinded fashion to find the sensitivity and specificity of the PET-PCR compared to the nested 18S rRNA PCR.
resultsCompared to microscopy, the PET-PCR assay was 59% more sensitive in detecting P. falciparum infections. The observed sensitivity and specificity were 100% (95% confidence interval (CI0.95) = 94-100%) and (CI0.95 = 96-100%), respectively, for the PET-PCR assay when compared to nested 18S rRNA PCR. When compared to 18S rRNA PCR, microscopy had a low sensitivity of 40% (CI0.95 = 23-61%) and specificity of 100% (CI0.95 = 96-100%). The PET-PCR results performed in the field laboratory in Tanzania were in 100% concordance with the results obtained at the reference laboratory in the USA.
conclusionThe PET-PCR is a new molecular diagnostic tool with similar performance characteristics as commonly used PCR methods that is less expensive, easy to use, and amiable to large scale-surveillance studies in developing country settings.
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