ArticleAmerican journal of respiratory and critical care medicine2014
Conditions associated with the cystic fibrosis defect promote chronic Pseudomonas aeruginosa infection.
Article in American journal of respiratory and critical care medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 128 citations in OpenAlex.
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- ChronicInternational journal of molecular sciences · 2025Article
- Survival strategies of imipenem-resistant P. aeruginosa: a comparative analysis of surface-bound biofilms and unbound aggregates.The Journal of antibiotics · 2025Article
- Flagellar motility and the mucus environment influence aggregation-mediated antibiotic tolerance ofmBio · 2025Article
- Outer membrane permeability ofMicrobiology spectrum · 2025Article
- Systematic bacteriophage selection for the lysis of multipleFrontiers in cellular and infection microbiology · 2025Article
- Lack of correlation betweenHeliyon · 2024Article
- Mucus polymer concentration andmBio · 2024Article
- Biochemical assessment in a cohort of pediatric patients with cystic fibrosis.Journal of medicine and life · 2024Article
- Biofilm antimicrobial susceptibility testing: where are we and where could we be going?Clinical microbiology reviews · 2023Review
- Nanoparticle-Mediated Strategies for Enhanced Drug Penetration and Retention in the Airway Mucosa.Pharmaceutics · 2023Review
- The non-attached biofilm aggregate.Communications biology · 2023Review
- Interactions between Bacteria andJournal of fungi (Basel, Switzerland) · 2023Review
- Pseudomonas aeruginosa type IV pili actively induce mucus contraction to form biofilms in tissue-engineered human airways.PLoS biology · 2023Article
- Distinct types of multicellular aggregates in Pseudomonas aeruginosa liquid cultures.NPJ biofilms and microbiomes · 2023Article
- Article
- Polysaccharides' Structures and Functions in Biofilm Architecture of Antimicrobial-Resistant (AMR) Pathogens.International journal of molecular sciences · 2023Review
- Transcriptomic and metabolomic analyses to study the key role by whichFrontiers in microbiology · 2023Article
26 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
Abstract
rationaleProgress has been made in understanding how the cystic fibrosis (CF) basic defect produces lung infection susceptibility. However, it remains unclear why CF exclusively leads to chronic infections that are noninvasive and highly resistant to eradication. Although biofilm formation has been suggested as a mechanism, recent work raises questions about the role of biofilms in CF.
objectivesTo learn how airway conditions attributed to CF transmembrane regulator dysfunction could lead to chronic infection, and to determine if biofilm-inhibiting genetic adaptations that are common in CF isolates affect the capacity of Pseudomonas aeruginosa to develop chronic infection phenotypes.
methodsWe studied P. aeruginosa isolates grown in agar and mucus gels containing sputum from patients with CF and measured their susceptibility to killing by antibiotics and host defenses. We also measured the invasive virulence of P. aeruginosa grown in sputum gels using airway epithelial cells and a murine infection model. MEASUREMENTS AND MAIN
resultsWe found that conditions likely to result from increased mucus density, hyperinflammation, and defective bacterial killing could all cause P. aeruginosa to grow in bacterial aggregates. Aggregated growth markedly increased the resistance of bacteria to killing by host defenses and antibiotics, and reduced their invasiveness. In addition, we found that biofilm-inhibiting mutations do not impede aggregate formation in gel growth environments.
conclusionsOur findings suggest that conditions associated with several CF pathogenesis hypotheses could cause the noninvasive and resistant infection phenotype, independently of the bacterial functions needed for biofilm formation.
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