Evidence map›Paper›PMID 24466488›Full record

ArticleBiomedical optics express2013

Pulsetrain-burst mode, ultrafast-laser interactions with 3D viable cell cultures as a model for soft biological tissues.

Zuoming Qian, Aghapi Mordovanakis, Joshua E Schoenly, Andrés Covarrubias, Yuanfeng Feng, Lothar Lilge, Robin S Marjoribanks

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomedical optics express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Zuoming QianDepartment of Physics, & Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto ON M5S 1A7, Canada.
Aghapi MordovanakisDepartment of Physics, & Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto ON M5S 1A7, Canada ; Current address: Biomedical Engineering, University of Michigan, 1101 Beal Avenue, Ann Arbor, Michigan 48109, USA.
Joshua E SchoenlyDepartment of Physics, & Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto ON M5S 1A7, Canada.
Andrés CovarrubiasDepartment of Physics, & Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto ON M5S 1A7, Canada.
Yuanfeng FengDepartment of Physics, & Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto ON M5S 1A7, Canada.
Lothar LilgePrincess Margaret Hospital, Ontario Cancer Institute and Department of Medical Biophysics, University of Toronto, 610 University Ave., Toronto ON M5G 2M9, Canada.
Robin S MarjoribanksDepartment of Physics, & Institute for Optical Sciences, University of Toronto, 60 St. George Street, Toronto ON M5S 1A7, Canada.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A 3D living-cell culture in hydrogel has been developed as a standardized low-tensile-strength tissue proxy for study of ultrafast, pulsetrain-burst laser-tissue interactions. The hydrogel is permeable to fluorescent biomarkers and optically transparent, allowing viable and necrotic cells to be imaged in 3D by confocal microscopy. Good cell-viability allowed us to distinguish between typical cell mortality and delayed subcellular tissue damage (e.g., apoptosis and DNA repair complex formation), caused by laser irradiation. The range of necrosis depended on laser intensity, but not on pulsetrain-burst duration. DNA double-strand breaks were quantified, giving a preliminary upper limit for genetic damage following laser treatment.

Indexed as

(140.3440) Laser-induced breakdown(160.1435) Biomaterials(170.1020) Ablation of tissue(170.2520) Fluorescence microscopy(320.7090) Ultrafast lasers(350.3390) Laser materials processing

Identifiers

PMID24466488
PMCPMC3891333

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.