ArticlePloS one2014
Identification and characterization of microRNAs in ovary and testis of Nile tilapia (Oreochromis niloticus) by using solexa sequencing technology.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 89 citations in OpenAlex.
- Gene regulation by non-Coding RNAs in infertility: a mechanistic review.Journal of ovarian research · 2025Review
- Sexual Antagonism and Sex Determination in Three Syngnathid Species Alongside a Male Pregnancy Gradient.Genome biology and evolution · 2025Article
- Identification of sex-biased MiRNA markers informative of heat-past events.BMC genomics · 2025Article
- Differential Expression of miRNAs, lncRNAs, and circRNAs between Ovaries and Testes in Common Carp (Cells · 2023Article
- Identification and Characterization of Sex-Biased miRNAs in the Golden Pompano (Animals : an open access journal from MDPI · 2022Article
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- Identification and expression analysis of sex biased miRNAs in chinese hook snout carpFrontiers in genetics · 2022Article
- Analysis of circRNA and miRNA expression profiles in IGF3-induced ovarian maturation in spotted scat (Frontiers in endocrinology · 2022Article
- Small RNA sequencing reveals sex-related miRNAs inFrontiers in genetics · 2022Article
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- Integrated Analysis of miR-430 on Steroidogenesis-Related Gene Expression of Larval Rice Field EelInternational journal of molecular sciences · 2021Article
- Identification of sex differentiation-related microRNA and long non-coding RNA in Takifugu rubripes gonads.Scientific reports · 2021Article
- Non-coding RNA Expression Patterns of Two Different Teleost Gonad Maturation Stages.Marine biotechnology (New York, N.Y.) · 2020Article
- Identification and Comparison of microRNAs in the Gonad of the Yellowfin Seabream (International journal of molecular sciences · 2020Article
- miRTil: An Extensive Repository for Nile Tilapia microRNA Next Generation Sequencing Data.Cells · 2020Article
- Heterogenic Origin of Micro RNAs in Atlantic Salmon (International journal of molecular sciences · 2020Article
- Selection of suitable candidate genes for miRNA expression normalization in Yellow River Carp (Cyprinus carpio. var).Scientific reports · 2019Article
- Comparative expression analysis of let-7 microRNAs during ovary development in Megalobrama amblycephala.Fish physiology and biochemistry · 2019Article
- MicroRNA Alternations in the Testes Related to the Sterility of Triploid Fish.Marine biotechnology (New York, N.Y.) · 2018Article
- Comprehensive Analysis of MicroRNA⁻Messenger RNA from White Yak Testis Reveals the Differentially Expressed Molecules Involved in Development and Reproduction.International journal of molecular sciences · 2018Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are endogenous non-coding small RNAs which play important roles in the regulation of gene expression by cleaving or inhibiting the translation of target gene transcripts. Thereinto, some specific miRNAs show regulatory activities in gonad development via translational control. In order to further understand the role of miRNA-mediated posttranscriptional regulation in Nile tilapia (Oreochromis niloticus) ovary and testis, two small RNA libraries of Nile tilapia were sequenced by Solexa small RNA deep sequencing methods. A total of 9,731,431 and 8,880,497 raw reads, representing 5,407,800 and 4,396,281 unique sequences were obtained from the sexually mature ovaries and testes, respectively. After comparing the small RNA sequences with the Rfam database, 1,432,210 reads in ovaries and 984,146 reads in testes were matched to the genome sequence of Nile tilapia. Bioinformatic analysis identified 764 mature miRNA, 209 miRNA-5p and 202 miRNA-3p were found in the two libraries, of which 525 known miRNAs are both expressed in the ovary and testis of Nile tilapia. Comparison of expression profiles of the testis, miR-727, miR-129 and miR-29 families were highly expressed in tilapia ovary. Additionally, miR-132, miR-212, miR-33a and miR-135b families, showed significant higher expression in testis compared with that in ovary. Furthermore, the expression patterns of the miRNAs were analyzed in different developmental stages of gonad. The result showed different expression patterns were observed during development of testis and ovary. In addition, the identification and characterization of differentially expressed miRNAs in the ovaries and testis of Nile tilapia provides important information on the role of miRNA in the regulation of the ovarian and testicular development and function. This data will be helpful to facilitate studies on the regulation of miRNAs during teleosts reproduction.
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