ArticlePloS one2014
Evaluation of the prevalence and production of Escherichia coli common pilus among avian pathogenic E. coli and its role in virulence.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Antimicrobial resistance and virulence gene profiles of Escherichia coli isolated from poultry farms using One Health perspective in Abeokuta, Nigeria.BMC microbiology · 2025Article
- Involvement of adhesins (EcpD, FdeC, FimH) expressed in mammary pathogenic Escherichia coli on adhesion to bovine mammary epithelial cells.Antonie van Leeuwenhoek · 2024Article
- Genomic diversity, pathogenicity and antimicrobial resistance of Escherichia coli isolated from poultry in the southern United States.BMC microbiology · 2023Article
- Comparative genomic analysis of uropathogenicFrontiers in microbiology · 2023Article
- Dietary Eugenol Nanoemulsion Potentiated Performance of Broiler Chickens: Orchestration of Digestive Enzymes, Intestinal Barrier Functions and Cytokines Related Gene Expression With a Consequence of Attenuating the Severity ofFrontiers in veterinary science · 2022Article
- Factors associated with Salmonella enterica and Escherichia coli during downtime in commercial broiler chicken barns in Ontario.Poultry science · 2021Article
- Novel Avian Pathogenic Escherichia coli Genes Responsible for Adhesion to Chicken and Human Cell Lines.Applied and environmental microbiology · 2020Article
- Protection against avian pathogenic Escherichia coli and Salmonella Kentucky exhibited in chickens given both probiotics and live Salmonella vaccine.Poultry science · 2020Article
- Bacteriophage activity against and characterisation of avian pathogenic Escherichia coli isolated from colibacillosis cases in Uganda.PloS one · 2020Article
- Virulence and antibiotic resistance profile of avianVeterinary world · 2019Article
- Pathogenic potential and the role of clones and plasmids in beta-lactamase-producing E. coli from chicken faeces in Vietnam.BMC veterinary research · 2019Article
- Article
- Novel small molecule modulators of quorum sensing in avian pathogenic Escherichia coli (APEC).Virulence · 2018Article
- Characterization of Spleen Transcriptome and Immunity Against Avian Colibacillosis After Immunization With Recombinant AttenuatedFrontiers in veterinary science · 2018Article
- Distribution of Major Pilin Subunit Genes Among Atypical EnteropathogenicFrontiers in microbiology · 2018Article
- A recombinant multi-antigen vaccine with broad protection potential against avian pathogenic Escherichia coli.PloS one · 2017Article
- Evaluation of Recombinant AttenuatedFrontiers in immunology · 2017Article
- FNR Regulates the Expression of Important Virulence Factors Contributing to the Pathogenicity of Avian PathogenicFrontiers in cellular and infection microbiology · 2017Article
- Novel vaccine antigen combinations elicit protective immune responses against Escherichia coli sepsis.Vaccine · 2016Article
- The arsenal of pathogens and antivirulence therapeutic strategies for disarming them.Drug design, development and therapy · 2016Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Avian pathogenic Escherichia coli (APEC) strains cause systemic and localized infections in poultry, jointly termed colibacillosis. Avian colibacillosis is responsible for significant economic losses to the poultry industry due to disease treatment, decrease in growth rate and egg production, and mortality. APEC are also considered a potential zoonotic risk for humans. Fully elucidating the virulence and zoonotic potential of APEC is key for designing successful strategies against their infections and their transmission. Herein, we investigated the prevalence of a newly discovered E. coli common pilus (ECP) for the subunit protein of the ECP pilus (ecpA) and ECP expression amongst APEC strains as well as the role of ECP in virulence. A PCR-based ecpA survey of a collection of 167 APEC strains has shown that 76% (127/167) were ecpA+. An immunofluorescence assay using anti-EcpA antibodies, revealed that among the ecpA+ strains, 37.8% (48/127) expressed ECP when grown in DMEM +0.5% Mannose in contact with HeLa cells at 37°C and/or in biofilm at 28°C; 35.4% (17/48) expressed ECP in both conditions and 64.6% (31/48) expressed ECP in biofilm only. We determined that the ecp operon in the APEC strain χ7122 (ecpA+, ECP-) was not truncated; the failure to detect ECP in some strains possessing non-truncated ecp genes might be attributed to differential regulatory mechanisms between strains that respond to specific environmental signals. To evaluate the role of ECP in the virulence of APEC, we generated ecpA and/or ecpD-deficient mutants from the strain χ7503 (ecpA+, ECP+). Deletion of ecpA and/or ecpD abolished ECP synthesis and expression, and reduced biofilm formation and motility in vitro and virulence in vivo. All together our data show that ecpA is highly prevalent among APEC isolates and its expression could be differentially regulated in these strains, and that ECP plays a role in the virulence of APEC.
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