ArticlePloS one2014
Increased metabolite levels of glycolysis and pentose phosphate pathway in rabbit atherosclerotic arteries and hypoxic macrophage.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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31 citing papers in PubMed, 79 citations in OpenAlex.
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- Inflammatory stimuli and hypoxia on atherosclerotic plaque thrombogenicity: Linking macrophage tissue factor and glycolysis.PloS one · 2025Article
- Energy metabolism in cardiovascular diseases: unlocking the hidden powerhouse of cardiac pathophysiology.Frontiers in endocrinology · 2025Review
- Targeting Lactate: An Emerging Strategy for Macrophage Regulation in Chronic Inflammation and Cancer.Biomolecules · 2024Review
- Steady Laminar Flow Decreases Endothelial Glycolytic Flux While Enhancing Proteoglycan Synthesis and Antioxidant Pathways.International journal of molecular sciences · 2024Article
- Defining the metabolic signatures associated with human macrophage polarisation.Biochemical Society transactions · 2023Article
- Advances in Glycolysis Metabolism of Atherosclerosis.Journal of cardiovascular translational research · 2023Review
- Bulk and single-cell characterisation of the immune heterogeneity of atherosclerosis identifies novel targets for immunotherapy.BMC biology · 2023Article
- Metabolism in atherosclerotic plaques: immunoregulatory mechanisms in the arterial wall.Clinical science (London, England : 1979) · 2022Review
- Metabolism of tissue macrophages in homeostasis and pathology.Cellular & molecular immunology · 2022Review
- Differential expression pattern, bioinformatics analysis, and validation of circRNA and mRNA in patients with arteriosclerosis.Frontiers in cardiovascular medicine · 2022Article
- Upregulated Kynurenine Pathway Enzymes in Aortic Atherosclerotic Aneurysm: Macrophage Kynureninase Downregulates Inflammation.Journal of atherosclerosis and thrombosis · 2021Article
- Atherosclerosis Impairs Naive CD4 T-Cell Responses via Disruption of Glycolysis.Arteriosclerosis, thrombosis, and vascular biology · 2021Article
- Potential role for pyruvate kinase M2 in the regulation of murine cardiac glycolytic flux during in vivo chronic hypoxia.Bioscience reports · 2021Article
- G6PD activity contributes to the regulation of histone acetylation and gene expression in smooth muscle cells and to the pathogenesis of vascular diseases.American journal of physiology. Heart and circulatory physiology · 2021Article
- A longitudinalCardiovascular diagnosis and therapy · 2021Article
- Article
- Polarization of Macrophages in Insects: Opening Gates for Immuno-Metabolic Research.Frontiers in cell and developmental biology · 2021Review
- Metabolic Consequences of Efferocytosis and its Impact on Atherosclerosis.Immunometabolism · 2021Article
- Myeloid Cell Diversity and Impact of Metabolic Cues during Atherosclerosis.Immunometabolism · 2020Article
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Authors and funding
14 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsInflammation and possibly hypoxia largely affect glucose utilization in atherosclerotic arteries, which could alter many metabolic systems. However, metabolic changes in atherosclerotic plaques remain unknown. The present study aims to identify changes in metabolic systems relative to glucose uptake and hypoxia in rabbit atherosclerotic arteries and cultured macrophages.
methodsMacrophage-rich or smooth muscle cell (SMC)-rich neointima was created by balloon injury in the iliac-femoral arteries of rabbits fed with a 0.5% cholesterol diet or a conventional diet. THP-1 macrophages stimulated with lipopolysaccharides (LPS) and interferon-γ (INFγ) were cultured under normoxic and hypoxic conditions. We evaluated comprehensive arterial and macrophage metabolism by performing metabolomic analyses using capillary electrophoresis-time of flight mass spectrometry. We evaluated glucose uptake and its relationship to vascular hypoxia using (18)F-fluorodeoxyglucose ((18)F-FDG) and pimonidazole, a marker of hypoxia.
resultsThe levels of many metabolites increased in the iliac-femoral arteries with macrophage-rich neointima, compared with those that were not injured and those with SMC-rich neointima (glycolysis, 4 of 9; pentose phosphate pathway, 4 of 6; tricarboxylic acid cycle, 4 of 6; nucleotides, 10 of 20). The uptake of (18)F-FDG in arterial walls measured by autoradiography positively correlated with macrophage- and pimonidazole-immunopositive areas (r = 0.76, and r = 0.59 respectively; n = 69 for both; p<0.0001). Pimonidazole immunoreactivity was closely localized with the nuclear translocation of hypoxia inducible factor-1α and hexokinase II expression in macrophage-rich neointima. The levels of glycolytic (8 of 8) and pentose phosphate pathway (4 of 6) metabolites increased in LPS and INFγ stimulated macrophages under hypoxic but not normoxic condition. Plasminogen activator inhibitor-1 protein levels in the supernatant were closely associated with metabolic pathways in the macrophages.
conclusionInfiltrative macrophages in atherosclerotic arteries might affect metabolic systems, and hypoxia but not classical activation might augment glycolytic and pentose phosphate pathways in macrophages.
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