ArticlePloS one2014
RNA-Seq analysis provides insights for understanding photoautotrophic polyhydroxyalkanoate production in recombinant Synechocystis Sp.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 38 citations in OpenAlex.
- Role of natural transformation in the evolution of small cryptic plasmids in Synechocystis sp. PCC 6803.Environmental microbiology reports · 2023Article
- Cyanobacteria as a Promising Alternative for Sustainable Environment: Synthesis of Biofuel and Biodegradable Plastics.Frontiers in microbiology · 2022Review
- Algal bioplastics: current market trends and technical aspects.Clean technologies and environmental policy · 2022Review
- Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes inmSphere · 2021Article
- Challenges and Perspectives of Polyhydroxyalkanoate Production From Microalgae/Cyanobacteria and Bacteria as Microbial Factories: An Assessment of Hybrid Biological System.Frontiers in bioengineering and biotechnology · 2021Review
- Maximizing PHB content in Synechocystis sp. PCC 6803: a new metabolic engineering strategy based on the regulator PirC.Microbial cell factories · 2020Article
- Article
- Genomic Survey of Salt Acclimation-Related Genes in the Halophilic Cyanobacterium Euhalothece sp. Z-M001.Scientific reports · 2020Article
- PHB is Produced from Glycogen Turn-over during Nitrogen Starvation inInternational journal of molecular sciences · 2019Article
- Bioprocess Engineering Aspects of Sustainable Polyhydroxyalkanoate Production in Cyanobacteria.Bioengineering (Basel, Switzerland) · 2018Review
- Novel quantitative insights into carbon sources for synthesis of poly hydroxybutyrate in Synechocystis PCC 6803.Photosynthesis research · 2018Article
- Ethylene causes transcriptomic changes inPlant direct · 2018Article
- Structure of polyhydroxyalkanoate (PHA) synthase PhaC from Chromobacterium sp. USM2, producing biodegradable plastics.Scientific reports · 2017Article
- Biosynthetic studies on terpenoids produced by Streptomyces.The Journal of antibiotics · 2017Review
- Cyanobacteria: Photoautotrophic Microbial Factories for the Sustainable Synthesis of Industrial Products.BioMed research international · 2015Review
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The photosynthetic cyanobacterium, Synechocystis sp. strain 6803, is a potential platform for the production of various chemicals and biofuels. In this study, direct photosynthetic production of a biopolymer, polyhydroxyalkanoate (PHA), in genetically engineered Synechocystis sp. achieved as high as 14 wt%. This is the highest production reported in Synechocystis sp. under photoautotrophic cultivation conditions without the addition of a carbon source. The addition of acetate increased PHA accumulation to 41 wt%, and this value is comparable to the highest production obtained with cyanobacteria. Transcriptome analysis by RNA-seq coupled with real-time PCR was performed to understand the global changes in transcript levels of cells subjected to conditions suitable for photoautotrophic PHA biosynthesis. There was lower expression of most PHA synthesis-related genes in recombinant Synechocystis sp. with higher PHA accumulation suggesting that the concentration of these enzymes is not the limiting factor to achieving high PHA accumulation. In order to cope with the higher PHA production, cells may utilize enhanced photosynthesis to drive the product formation. Results from this study suggest that the total flux of carbon is the possible driving force for the biosynthesis of PHA and the polymerizing enzyme, PHA synthase, is not the only critical factor affecting PHA-synthesis. Knowledge of the regulation or control points of the biopolymer production pathways will facilitate the further use of cyanobacteria for biotechnological applications.
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