ArticlePloS one2014
A role for topographic cues in the organization of collagenous matrix by corneal fibroblasts and stem cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Interleukin-1 and Transforming Growth Factor Beta: Commonly Opposing, but Sometimes Supporting, Master Regulators of the Corneal Wound Healing Response to Injury.Investigative ophthalmology & visual science · 2021Pooled it
- A Microwell Platform for Characterizing the Dynamic Response of Corneal Keratocytes to Biochemical and Biophysical Cues.Micromachines · 2026Article
- Keratin-Laden Bioink for Corneal Stroma Bioprinting.Bioengineering (Basel, Switzerland) · 2026Article
- Single cell RNA-seq characterization of non-fibrotic stromal wound repopulation in the rabbit.Experimental eye research · 2026Article
- Directed Differentiation of Adipose-Derived Stem Cells Using Imprinted Cell-Like Topographies as a Growth Factor-Free Approach.Stem cell reviews and reports · 2024Review
- Focal Adhesion Maturation Responsible for Behavioral Changes in Human Corneal Stromal Fibroblasts on Fibrillar Substrates.International journal of molecular sciences · 2024Article
- Squishy matters - Corneal mechanobiology in health and disease.Progress in retinal and eye research · 2024Review
- Fabrication of Micropatterns of Aligned Collagen Fibrils.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Posterior corneoscleral limbus: Architecture, stem cells, and clinical implications.Progress in retinal and eye research · 2023Review
- Regenerative therapy for the Cornea.Progress in retinal and eye research · 2022Review
- Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing.International journal of molecular sciences · 2022Article
- Advances in Regulatory Strategies of Differentiating Stem Cells towards Keratocytes.Stem cells international · 2022Review
- Mimicking the Human Tympanic Membrane: The Significance of Scaffold Geometry.Advanced healthcare materials · 2021Article
- The Human Tissue-Engineered Cornea (hTEC): Recent Progress.International journal of molecular sciences · 2021Review
- Methods for Investigating Corneal Cell Interactions and Extracellular Vesicles In Vitro.Current protocols in cell biology · 2020Article
- Keratocyte mechanobiology.Experimental eye research · 2020Review
- Engineering a Corneal Stromal Equivalent Using a Novel Multilayered Fabrication Assembly Technique.Tissue engineering. Part A · 2020Article
- Corneal epithelial biology: Lessons stemming from old to new.Experimental eye research · 2020Review
- Hypothermically Stored Adipose-Derived Mesenchymal Stromal Cell Alginate Bandages Facilitate Use of Paracrine Molecules for Corneal Wound Healing.International journal of molecular sciences · 2020Article
- A high-throughput microfluidic method for fabricating aligned collagen fibrils to study Keratocyte behavior.Biomedical microdevices · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Human corneal fibroblasts (HCF) and corneal stromal stem cells (CSSC) each secrete and organize a thick stroma-like extracellular matrix in response to different substrata, but neither cell type organizes matrix on tissue-culture polystyrene. This study compared cell differentiation and extracellular matrix secreted by these two cell types when they were cultured on identical substrata, polycarbonate Transwell filters. After 4 weeks in culture, both cell types upregulated expression of genes marking differentiated keratocytes (KERA, CHST6, AQP1, B3GNT7). Absolute expression levels of these genes and secretion of keratan sulfate proteoglycans were significantly greater in CSSC than HCF. Both cultures produced extensive extracellular matrix of aligned collagen fibrils types I and V, exhibiting cornea-like lamellar structure. Unlike HCF, CSSC produced little matrix in the presence of serum. Construct thickness and collagen organization was enhanced by TGF-ß3. Scanning electron microscopic examination of the polycarbonate membrane revealed shallow parallel grooves with spacing of 200-300 nm, similar to the topography of aligned nanofiber substratum which we previously showed to induce matrix organization by CSSC. These results demonstrate that both corneal fibroblasts and stromal stem cells respond to a specific pattern of topographical cues by secreting highly organized extracellular matrix typical of corneal stroma. The data also suggest that the potential for matrix secretion and organization may not be directly related to the expression of molecular markers used to identify differentiated keratocytes.
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Registered trials
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