Evidence map›Paper›PMID 24465845›Full record

ArticlePloS one2014

Semi-automated, occupationally safe immunofluorescence microtip sensor for rapid detection of Mycobacterium cells in sputum.

Shinnosuke Inoue, Annie L Becker, Jong-Hoon Kim, Zhiquan Shu, Scott D Soelberg, Kris M Weigel, Morgan Hiraiwa, Andrew Cairns, Hyun-Boo Lee, Clement E Furlong and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Amperometric immunosensor for rapid detection of Mycobacterium tuberculosis.Journal of micromechanics and microengineering : structures, devices, and systems
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Shinnosuke InoueDepartment of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America.
Annie L BeckerDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, United States of America.
Jong-Hoon KimDepartment of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America.
Zhiquan ShuDepartment of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America.
Scott D SoelbergDepartments of Medicine-Division of Medical Genetics and Genome Sciences, University of Washington, Seattle, Washington, United States of America.
Kris M WeigelDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, United States of America.
Morgan HiraiwaDepartment of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America.
Andrew CairnsDepartment of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America.
Hyun-Boo LeeDepartment of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America.
Clement E FurlongDepartments of Medicine-Division of Medical Genetics and Genome Sciences, University of Washington, Seattle, Washington, United States of America.
Kieseok OhNanoFacture, Inc., Bellevue, Washington, United States of America.
Kyong-Hoon LeeNanoFacture, Inc., Bellevue, Washington, United States of America.
Dayong GaoDepartment of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America.
Jae-Hyun ChungDepartment of Mechanical Engineering, University of Washington, Seattle, Washington, United States of America.
Gerard A CangelosiDepartment of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington, United States of America.

Funding

Point-of-care concentration and detection of pathogen antigens in samplesR01AI093418 · NIAID · UNIVERSITY OF WASHINGTON · PI CANGELOSI, GERARD A · 2011 to 2013
$1.2M
NIAID NIH HHS R01 AI093418
6 · The paper itself

Abstract

An occupationally safe (biosafe) sputum liquefaction protocol was developed for use with a semi-automated antibody-based microtip immunofluorescence sensor. The protocol effectively liquefied sputum and inactivated microorganisms including Mycobacterium tuberculosis, while preserving the antibody-binding activity of Mycobacterium cell surface antigens. Sputum was treated with a synergistic chemical-thermal protocol that included moderate concentrations of NaOH and detergent at 60°C for 5 to 10 min. Samples spiked with M. tuberculosis complex cells showed approximately 10(6)-fold inactivation of the pathogen after treatment. Antibody binding was retained post-treatment, as determined by analysis with a microtip immunosensor. The sensor correctly distinguished between Mycobacterium species and other cell types naturally present in biosafe-treated sputum, with a detection limit of 100 CFU/mL for M. tuberculosis, in a 30-minute sample-to-result process. The microtip device was also semi-automated and shown to be compatible with low-cost, LED-powered fluorescence microscopy. The device and biosafe sputum liquefaction method opens the door to rapid detection of tuberculosis in settings with limited laboratory infrastructure.

Indexed as

Biosensing TechniquesFluorescent Antibody TechniqueHost-Pathogen InteractionsHumansLab-On-A-Chip DevicesMicrochip Analytical ProceduresMicroscopy, FluorescenceMycobacterium tuberculosisOccupational HealthReproducibility of ResultsSensitivity and SpecificitySputumTime FactorsTuberculosis

Identifiers

PMID24465845
PMCPMC3899086

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.