ArticlePloS one2014
Physiological normoxia and absence of EGF is required for the long-term propagation of anterior neural precursors from human pluripotent cells.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed.
- Striatal neuron dysfunction in C9ORF72-FTD/ALS is driven by AIS and potassium channel dysregulation.Cell reports · 2026Article
- NK-cell cytotoxicity toward pluripotent stem cells and their neural progeny: impacts of activating and inhibitory receptors and KIR/HLA mismatch.Stem cells (Dayton, Ohio) · 2025Article
- Reproducible Differentiation of Human Pluripotent Stem Cells into Two-Dimensional Cortical Neuron Cultures with Checkpoints for Success.Current protocols · 2023Article
- Signal requirement for cortical potential of transplantable human neuroepithelial stem cells.Nature communications · 2022Article
- Article
- Postsynaptic structure formation of human iPS cell-derived neurons takes longer than presynaptic formation during neural differentiation in vitro.Molecular brain · 2021Article
- iPSC-derived myelinoids to study myelin biology of humans.Developmental cell · 2021Article
- Altered network properties in C9ORF72 repeat expansion cortical neurons are due to synaptic dysfunction.Molecular neurodegeneration · 2021Article
- Cortical neurons derived from human pluripotent stem cells lacking FMRP display altered spontaneous firing patterns.Molecular autism · 2020Article
- Article
- Mutant C9orf72 human iPSC-derived astrocytes cause non-cell autonomous motor neuron pathophysiology.Glia · 2020Article
- Neuronal Activity and Its Role in Controlling Antioxidant Genes.International journal of molecular sciences · 2020Review
- L-type voltage-gated calcium channel regulation of in vitro human cortical neuronal networks.Scientific reports · 2019Article
- CellSIUS provides sensitive and specific detection of rare cell populations from complex single-cell RNA-seq data.Genome biology · 2019Article
- Efficient Neural Differentiation of hPSCs by Extrinsic Signals Derived from Co-cultured Neural Stem or Precursor Cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2019Article
- The effect of rho kinase inhibition on morphological and electrophysiological maturity in iPSC-derived neurons.Cell and tissue research · 2019Article
- DISC1 regulates N-methyl-D-aspartate receptor dynamics: abnormalities induced by a Disc1 mutation modelling a translocation linked to major mental illness.Translational psychiatry · 2018Article
- C9ORF72 repeat expansion causes vulnerability of motor neurons to CaNature communications · 2018Article
- Adherent vs. Free-Floating Neural Induction by Dual SMAD Inhibition for Neurosphere Cultures Derived from Human Induced Pluripotent Stem Cells.Frontiers in cell and developmental biology · 2018Article
- Neurons and neuronal activity control gene expression in astrocytes to regulate their development and metabolism.Nature communications · 2017Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Widespread use of human pluripotent stem cells (hPSCs) to study neuronal physiology and function is hindered by the ongoing need for specialist expertise in converting hPSCs to neural precursor cells (NPCs). Here, we describe a new methodology to generate cryo-preservable hPSC-derived NPCs that retain an anterior identity and are propagatable long-term prior to terminal differentiation, thus abrogating regular de novo neuralization. Key to achieving passagable NPCs without loss of identity is the combination of both absence of EGF and propagation in physiological levels (3%) of O2. NPCs generated in this way display a stable long-term anterior forebrain identity and importantly retain developmental competence to patterning signals. Moreover, compared to NPCs maintained at ambient O2 (21%), they exhibit enhanced uniformity and speed of functional maturation, yielding both deep and upper layer cortical excitatory neurons. These neurons display multiple attributes including the capability to form functional synapses and undergo activity-dependent gene regulation. The platform described achieves long-term maintenance of anterior neural precursors that can give rise to forebrain neurones in abundance, enabling standardised functional studies of neural stem cell maintenance, lineage choice and neuronal functional maturation for neurodevelopmental research and disease-modelling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.