ArticlePloS one2014
Reliable single cell array CGH for clinical samples.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Micrometastasis-derived models enable drug testing for early-stage, high-risk melanoma patients.EMBO molecular medicine · 2026Article
- Array comparative genomic hybridisation in haematological malignancies: A comprehensive review.African journal of laboratory medicine · 2026Review
- Single-Cell Analysis of Bone-Marrow-Disseminated Tumour Cells.Diagnostics (Basel, Switzerland) · 2024Review
- Array-Based Comparative Genomic Hybridization for the Detection of Copy Number Alterations in Single Cells.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Simultaneous Isolation and Amplification of mRNA and Genomic DNA of a Single Cell.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Retrospective cell lineage reconstruction in humans by using short tandem repeats.Cell reports methods · 2021Article
- Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities.Cancers · 2021Review
- Assessing the performance of methods for copy number aberration detection from single-cell DNA sequencing data.PLoS computational biology · 2020Article
- Unravelling the biological secrets of microchimerism by single-cell analysis.Briefings in functional genomics · 2018Article
- Cancer Diagnosis Using a Liquid Biopsy: Challenges and Expectations.Diagnostics (Basel, Switzerland) · 2018Review
- Single cell polarity in liquid phase facilitates tumour metastasis.Nature communications · 2018Article
- Genetic alterations driving metastatic colony formation are acquired outside of the primary tumour in melanoma.Nature communications · 2018Article
- Article
- Genomic and expression profiling reveal molecular heterogeneity of disseminated tumor cells in bone marrow of early breast cancer.NPJ breast cancer · 2018Article
- Development of a test that measures real-time HER2 signaling function in live breast cancer cell lines and primary cells.BMC cancer · 2017Article
- Catch and Release: rare cell analysis from a functionalised medical wire.Scientific reports · 2017Article
- Tracing the origin of disseminated tumor cells in breast cancer using single-cell sequencing.Genome biology · 2016Article
- Approaches to isolation and molecular characterization of disseminated tumor cells.Oncotarget · 2015Review
- Review
- Molecular profiling of single circulating tumor cells with diagnostic intention.EMBO molecular medicine · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDisseminated cancer cells (DCCs) and circulating tumor cells (CTCs) are extremely rare, but comprise the precursors cells of distant metastases or therapy resistant cells. The detailed molecular analysis of these cells may help to identify key events of cancer cell dissemination, metastatic colony formation and systemic therapy escape. METHODOLOGY/PRINCIPAL
findingsUsing the Ampli1™ whole genome amplification (WGA) technology and high-resolution oligonucleotide aCGH microarrays we optimized conditions for the analysis of structural copy number changes. The protocol presented here enables reliable detection of numerical genomic alterations as small as 0.1 Mb in a single cell. Analysis of single cells from well-characterized cell lines and single normal cells confirmed the stringent quantitative nature of the amplification and hybridization protocol. Importantly, fixation and staining procedures used to detect DCCs showed no significant impact on the outcome of the analysis, proving the clinical usability of our method. In a proof-of-principle study we tracked the chromosomal changes of single DCCs over a full course of high-dose chemotherapy treatment by isolating and analyzing DCCs of an individual breast cancer patient at four different time points. CONCLUSIONS/SIGNIFICANCE: The protocol enables detailed genome analysis of DCCs and thereby assessment of the clonal evolution during the natural course of the disease and under selection pressures. The results from an exemplary patient provide evidence that DCCs surviving selective therapeutic conditions may be recruited from a pool of genomically less advanced cells, which display a stable subset of specific genomic alterations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.