ArticlePloS one2014
Scrutinizing virus genome termini by high-throughput sequencing.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
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- Whole genome sequence analysis of bacteriophage P1 that infects the Lactobacillus plantarum.Virus genes · 2022Article
- A temperateVirulence · 2022Article
- NovelViruses · 2022Article
- NovelViruses · 2022Article
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- Characterization and Genomic Analysis of Bacteriophage vB_KpnM_IME346 Targeting Clinical Klebsiella pneumoniae Strain of the K63 Capsular Type.Current microbiology · 2022Article
- High-throughput identification of viral termini and packaging mechanisms in virome datasets using PhageTermVirome.Scientific reports · 2021Article
- Isolation, Characterization, and Genome Sequence Analysis of a Novel Lytic Phage, Xoo-sp15 Infecting Xanthomonas oryzae pv. oryzae.Current microbiology · 2021Article
- vB_BcM_Sam46 and vB_BcM_Sam112, members of a new bacteriophage genus with unusual small terminase structure.Scientific reports · 2021Article
- Evaluation of Potential ARG Packaging by Two Environmental T7-Like Phage during Phage-Host Interaction.Viruses · 2020Article
- Article
- Characterization and genome analysis of a novel bacteriophage vB_SpuP_Spp16 that infects Salmonella enterica serovar pullorum.Virus genes · 2019Article
- Isolation of a T7-Like LyticViruses · 2019Article
- Discovery of Shiga Toxin-ProducingFrontiers in microbiology · 2019Article
- Article
- Novel N4-Like Bacteriophages of Pectobacterium atrosepticum.Pharmaceuticals (Basel, Switzerland) · 2018Article
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Analysis of genomic terminal sequences has been a major step in studies on viral DNA replication and packaging mechanisms. However, traditional methods to study genome termini are challenging due to the time-consuming protocols and their inefficiency where critical details are lost easily. Recent advances in next generation sequencing (NGS) have enabled it to be a powerful tool to study genome termini. In this study, using NGS we sequenced one iridovirus genome and twenty phage genomes and confirmed for the first time that the high frequency sequences (HFSs) found in the NGS reads are indeed the terminal sequences of viral genomes. Further, we established a criterion to distinguish the type of termini and the viral packaging mode. We also obtained additional terminal details such as terminal repeats, multi-termini, asymmetric termini. With this approach, we were able to simultaneously detect details of the genome termini as well as obtain the complete sequence of bacteriophage genomes. Theoretically, this application can be further extended to analyze larger and more complicated genomes of plant and animal viruses. This study proposed a novel and efficient method for research on viral replication, packaging, terminase activity, transcription regulation, and metabolism of the host cell.
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