ArticlePloS one2014
Noninvasive quantification of in vitro osteoblastic differentiation in 3D engineered tissue constructs using spectral ultrasound imaging.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Ultrasound-responsive composite hydrogels: Design rules for spatiotemporally controlled drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2026Review
- New Nanobioceramics Based on Hydroxyapatite for Biomedical Applications: Stability and Properties.Nanomaterials (Basel, Switzerland) · 2025Article
- Digital Light Processing ofMaterials (Basel, Switzerland) · 2024Article
- Echo intensity and gray-level co-occurrence matrix analysis of soft tissue grafting biomaterials and dental implants: anDento maxillo facial radiology · 2023Article
- Biological and Physico-Chemical Properties of Composite Layers Based on Magnesium-Doped Hydroxyapatite in Chitosan Matrix.Micromachines · 2022Article
- Therapeutic Ultrasound Effects on Human Induced Pluripotent Stem Cell Cardiomyocytes Measured Optically and with Spectral Ultrasound.Ultrasound in medicine & biology · 2022Article
- A novel quantitative and reference-free ultrasound analysis to discriminate different concentrations of bone mineral content.Scientific reports · 2021Article
- Injectable pre-cultured tissue modules catalyze the formation of extensive functional microvasculature in vivo.Scientific reports · 2020Article
- Photoacoustic Imaging in Tissue Engineering and Regenerative Medicine.Tissue engineering. Part B, Reviews · 2020Review
- High Frequency Spectral Ultrasound Imaging to Detect Metastasis in Implanted Biomaterial Scaffolds.Annals of biomedical engineering · 2020Article
- Injectable osteogenic microtissues containing mesenchymal stromal cells conformally fill and repair critical-size defects.Biomaterials · 2019Article
- Article
- Multimodal Label-Free Imaging for Detecting Maturation of Engineered Osteogenic Grafts.ACS biomaterials science & engineering · 2019Article
- Dispersible hydrogel force sensors reveal patterns of solid mechanical stress in multicellular spheroid cultures.Nature communications · 2019Article
- Multimode ultrasound viscoelastography for three-dimensional interrogation of microscale mechanical properties in heterogeneous biomaterials.Biomaterials · 2018Article
- Article
- New Bioengineering Breakthroughs and Enabling Tools in Regenerative Medicine.Current stem cell reports · 2017Review
- Integrating Concepts of Material Mechanics, Ligand Chemistry, Dimensionality and Degradation to Control Differentiation of Mesenchymal Stem Cells.Current opinion in solid state & materials science · 2016Article
- Ultrasound Imaging Techniques for Spatiotemporal Characterization of Composition, Microstructure, and Mechanical Properties in Tissue Engineering.Tissue engineering. Part B, Reviews · 2016Review
- Microscale characterization of the viscoelastic properties of hydrogel biomaterials using dual-mode ultrasound elastography.Biomaterials · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Non-destructive monitoring of engineered tissues is needed for translation of these products from the lab to the clinic. In this study, non-invasive, high resolution spectral ultrasound imaging (SUSI) was used to monitor the differentiation of MC3T3 pre-osteoblasts seeded within collagen hydrogels. SUSI was used to measure the diameter, concentration and acoustic attenuation of scatterers within such constructs cultured in either control or osteogenic medium over 21 days. Conventional biochemical assays were used on parallel samples to determine DNA content and calcium deposition. Construct volume and morphology were accurately imaged using ultrasound. Cell diameter was estimated to be approximately 12.5-15.5 µm using SUSI, which corresponded well to measurements of fluorescently stained cells. The total number of cells per construct assessed by quantitation of DNA content decreased from 5.6±2.4×10(4) at day 1 to 0.9±0.2×10(4) at day 21. SUSI estimation of the equivalent number of acoustic scatters showed a similar decreasing trend, except at day 21 in the osteogenic samples, which showed a marked increase in both scatterer number and acoustic impedance, suggestive of mineral deposition by the differentiating MC3T3 cells. Estimation of calcium content by SUSI was 41.7±11.4 µg/ml, which agreed well with the biochemical measurement of 38.7±16.7 µg/ml. Color coded maps of parameter values were overlaid on B-mode images to show spatiotemporal changes in cell diameter and calcium deposition. This study demonstrates the use of non-destructive ultrasound imaging to provide quantitative information on the number and differentiated state of cells embedded within 3D engineered constructs, and therefore presents a valuable tool for longitudinal monitoring of engineered tissue development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.