ArticlePloS one2014
Signal intensities derived from different NMR probes and parameters contribute to variations in quantification of metabolites.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed.
- Pharmacometabolomics of l-carnitine treatment response phenotypes in patients with septic shock.Annals of the American Thoracic Society · 2015Trial
- Gut microbiome-mediated nutrients alter opportunistic bacterial growth in peritonitis.American journal of physiology. Gastrointestinal and liver physiology · 2025Article
- Urine Metabolites of Suspected Community-Acquired Pneumonia.The Journal of infectious diseases · 2025Article
- Comparison and Determination of the Content of Mosapride Citrate by Different qNMR Methods.International journal of molecular sciences · 2024Article
- Metabolomics: An Emerging "Omics" Platform for Systems Biology and Its Implications for Huntington Disease Research.Metabolites · 2023Review
- Metabolomics and NMR.Handbook of experimental pharmacology · 2023Article
- Metabolomics of Respiratory Diseases.Handbook of experimental pharmacology · 2023Review
- Respiratory Colonization and Short-Term Temporal Changes in the Urinary Metabolome of Children.Metabolites · 2021Article
- Magnetic resonance spectroscopy for the study of cns malignancies.Progress in nuclear magnetic resonance spectroscopy · 2021Article
- Nuclear Magnetic Resonance Spectroscopy in Clinical Metabolomics and Personalized Medicine: Current Challenges and Perspectives.Frontiers in molecular biosciences · 2021Review
- Serum amino acid concentrations and clinical outcomes in smokers: SPIROMICS metabolomics study.Scientific reports · 2019Article
- NMR Spectroscopy for Metabolomics Research.Metabolites · 2019Review
- Rapid, Reproducible, Quantifiable NMR Metabolomics: Methanol and Methanol: Chloroform Precipitation for Removal of Macromolecules in Serum and Whole Blood.Metabolites · 2018Article
- Pharmacometabolomics reveals a role for histidine, phenylalanine, and threonine in the development of paclitaxel-induced peripheral neuropathy.Breast cancer research and treatment · 2018Article
- Atypical Antipsychotic Exposure May Not Differentiate Metabolic Phenotypes of Patients with Schizophrenia.Pharmacotherapy · 2018Article
- New Strategies and Challenges in Lung Proteomics and Metabolomics. An Official American Thoracic Society Workshop Report.Annals of the American Thoracic Society · 2017Article
- Emerging Biomarkers of Illness Severity: Urinary Metabolites Associated with Sepsis and Necrotizing Methicillin-Resistant Staphylococcus aureus Pneumonia.Pharmacotherapy · 2017Article
- Metabolomics as a Driver in Advancing Precision Medicine in Sepsis.Pharmacotherapy · 2017Review
- Macrophage-Mediated Clofazimine Sequestration Is Accompanied by a Shift in Host Energy Metabolism.Journal of pharmaceutical sciences · 2017Article
- Training in metabolomics research. I. Designing the experiment, collecting and extracting samples and generating metabolomics data.Journal of mass spectrometry : JMS · 2016Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors.
Funding
Abstract
We discovered that serious issues could arise that may complicate interpretation of metabolomic data when identical samples are analyzed at more than one NMR facility, or using slightly different NMR parameters on the same instrument. This is important because cross-center validation metabolomics studies are essential for the reliable application of metabolomics to clinical biomarker discovery. To test the reproducibility of quantified metabolite data at multiple sites, technical replicates of urine samples were assayed by 1D-(1)H-NMR at the University of Alberta and the University of Michigan. Urine samples were obtained from healthy controls under a standard operating procedure for collection and processing. Subsequent analysis using standard statistical techniques revealed that quantitative data across sites can be achieved, but also that previously unrecognized NMR parameter differences can dramatically and widely perturb results. We present here a confirmed validation of NMR analysis at two sites, and report the range and magnitude that common NMR parameters involved in solvent suppression can have on quantitated metabolomics data. Specifically, saturation power levels greatly influenced peak height intensities in a frequency-dependent manner for a number of metabolites, which markedly impacted the quantification of metabolites. We also investigated other NMR parameters to determine their effects on further quantitative accuracy and precision. Collectively, these findings highlight the importance of and need for consistent use of NMR parameter settings within and across centers in order to generate reliable, reproducible quantified NMR metabolomics data.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.