ArticlePloS one2014
RNA polymerase III transcriptomes in human embryonic stem cells and induced pluripotent stem cells, and relationships with pluripotency transcription factors.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 34 citations in OpenAlex.
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- A cancer-associated RNA polymerase III identity drives robust transcription and expression of snaR-A noncoding RNA.Nature communications · 2022Article
- Regulatory networking of the three RNA polymerases helps the eukaryotic cells cope with environmental stress.Current genetics · 2021Review
- RNA Polymerase III, Ageing and Longevity.Frontiers in genetics · 2021Review
- Chromatin Landscape Dynamics in the Early Development of the Plant Parasitic Nematode Meloidogyne incognita.Frontiers in cell and developmental biology · 2021Article
- Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans.Scientific reports · 2020Article
- Functions of paralogous RNA polymerase III subunits POLR3G and POLR3GL in mouse development.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- RNA polymerase III transcription as a disease factor.Genes & development · 2020Review
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- TDP-43 regulates transcription at protein-coding genes and Alu retrotransposons.Biochimica et biophysica acta. Gene regulatory mechanisms · 2019Article
- Leukodystrophy-associatedThe Journal of biological chemistry · 2019Article
- Differential regulation of RNA polymerase III genes during liver regeneration.Nucleic acids research · 2019Article
- Maf1 and Repression of RNA Polymerase III-Mediated Transcription Drive Adipocyte Differentiation.Cell reports · 2018Article
- Signaling to and from the RNA Polymerase III Transcription and Processing Machinery.Annual review of biochemistry · 2018Review
- Epigenetic regulation of RNA polymerase III transcription in early breast tumorigenesis.Oncogene · 2017Article
- Topological organization and dynamic regulation of human tRNA genes during macrophage differentiation.Genome biology · 2017Article
- Genome-wide mapping of infection-induced SINE RNAs reveals a role in selective mRNA export.Nucleic acids research · 2017Article
- RNA Polymerase III Subunit POLR3G Regulates Specific Subsets of PolyAStem cell reports · 2017Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
Abstract
Recent genomic approaches have revealed that the repertoire of RNA Pol III-transcribed genes varies in different human cell types, and that this variation is likely determined by a combination of the chromatin landscape, cell-specific DNA-binding transcription factors, and collaboration with RNA Pol II. Although much is known about this regulation in differentiated human cells, there is presently little understanding of this aspect of the Pol III system in human ES cells. Here, we determine the occupancy profiles of Pol III components in human H1 ES cells, and also induced pluripotent cells, and compare to known profiles of chromatin, transcription factors, and RNA expression. We find a relatively large fraction of the Pol III repertoire occupied in human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). In ES cells we find clear correlations between Pol III occupancy and active chromatin. Interestingly, we find a highly significant fraction of Pol III-occupied genes with adjacent binding events by pluripotency factors in ES cells, especially NANOG. Notably, in human ES cells we find H3K27me3 adjacent to but not overlapping many active Pol III loci. We observe in all such cases, a peak of H3K4me3 and/or RNA Pol II, between the H3K27me3 and Pol III binding peaks, suggesting that H3K4me3 and Pol II activity may "insulate" Pol III from neighboring repressive H3K27me3. Further, we find iPSCs have a larger Pol III repertoire than their precursors. Finally, the active Pol III genome in iPSCs is not completely reprogrammed to a hESC like state and partially retains the transcriptional repertoire of the precursor. Together, our correlative results are consistent with Pol III binding and activity in human ES cells being enabled by active/permissive chromatin that is shaped in part by the pluripotency network of transcription factors and RNA Pol II activity.
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