ArticlePloS one2014
Adverse effects from clenbuterol and ractopamine on nematode Caenorhabditis elegans and the underlying mechanism.
Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 55 citations in OpenAlex.
- A Review of the Literature on the Endocrine Disruptor Activity Testing of Bisphenols inJournal of xenobiotics · 2026Review
- Effects of feeding a Saccharomyces cerevisiae fermentation product and ractopamine hydrochloride to finishing beef steers on growth performance, immune system, and muscle gene expression.Journal of animal science · 2023Article
- Ractopamine at the Center of Decades-Long Scientific and Legal Disputes: A Lesson on Benefits, Safety Issues, and Conflicts.Biomolecules · 2022Review
- Chinese liquor extract attenuates oxidative damage in HepG2 cells and extends lifespan ofFood science & nutrition · 2020Article
- TheBiochemistry and biophysics reports · 2017Article
- Coal combustion related fine particulate matter (PMToxicology research · 2017Article
- Screening lifespan-extending drugs in Caenorhabditis elegans via label propagation on drug-protein networks.BMC systems biology · 2016Article
- Wnt Ligands Differentially Regulate Toxicity and Translocation of Graphene Oxide through Different Mechanisms in Caenorhabditis elegans.Scientific reports · 2016Article
- The Use of the Nematode Caenorhabditis elegans to Evaluate the Adverse Effects of Epoxiconazole Exposure on Spermatogenesis.International journal of environmental research and public health · 2016Article
- Metallothioneins act downstream of insulin signaling to regulate toxicity of outdoor fine particulate matter (PMToxicology research · 2016Article
- Anti-aging effect of polysaccharide from Bletilla striata on nematode Caenorhabditis elegans.Pharmacognosy magazineArticle
- The genetics of isoflurane-induced developmental neurotoxicity.Neurotoxicology and teratologyArticle
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the present study, we used Caenorhabditis elegans assay system to investigate in vivo toxicity from clentuberol and ractopamine and the possible underlying mechanism. Both acute and prolonged exposures to clentuberol or ractopamine decreased brood size and locomotion behavior, and induced intestinal autofluorescence and reactive oxygen species (ROS) production. Although acute exposure to the examined concentrations of clentuberol or ractopamine did not induce lethality, prolonged exposure to 10 µg/L of clentuberol and ractopamine reduced lifespan. At relatively high concentrations, ractopamine exhibited more severe toxicity than clentuberol on nematodes. Overexpression of sod-2 gene encoding a Mn-SOD to prevent induction of oxidative stress effectively inhibited toxicity from clentuberol or ractopamine. Besides oxidative stress, we found that clentuberol might reduce lifespan through influencing insulin/IGF signaling pathway; however, ractopamine might reduce lifespan through affecting both insulin/IGF signaling pathway and TOR signaling pathway. Ractopamine more severely decreased expression levels of daf-16, sgk-1, skn-1, and aak-2 genes than clentuberol, and increased expression levels of daf-2 and age-1 genes at the examined concentration. Therefore, the C. elegans assay system may be useful for assessing the possible toxicity from weight loss agents, and clentuberol and ractopamine may induce toxicity through different molecular mechanisms.
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