Evidence map›Paper›PMID 24465482›Full record

ArticlePloS one2014

Efficient reprogramming of naïve-like induced pluripotent stem cells from porcine adipose-derived stem cells with a feeder-independent and serum-free system.

Yu Zhang, Chao Wei, Pengfei Zhang, Xia Li, Tong Liu, Yong Pu, Yunsheng Li, Zubing Cao, Hongguo Cao, Ya Liu and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.

0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
3.3field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

27 citing papers in PubMed, 54 citations in OpenAlex.

  1. Review
  2. Article
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  6. Zoological research · 2022
    Article
  7. Review
  8. Review
  9. Article
  10. Review
  11. Review
  12. Article
  13. Induced pluripotent stem cells from farm animals.Journal of animal science · 2020
    Review
  14. Article
  15. Article
  16. Article
  17. Article
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  19. Article
  20. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Yu ZhangAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Chao WeiAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Pengfei ZhangAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Xia LiAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Tong LiuAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Yong PuAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Yunsheng LiAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Zubing CaoAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Hongguo CaoAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Ya LiuAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Xiaorong ZhangAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Yunhai ZhangAnhui Provincial Laboratory for Local Livestock and Poultry Genetic Resource Conservation and Bio-breeding, Department of Animal Science, College of Animal Science and Technology, Anhui Agricultural University, Hefei, Anhui, People's Republic of China.
Anhui Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Induced pluripotent stem cells (iPSCs) are somatic cells reprogrammed by ectopic expression of transcription factors or small molecule treatment, which resemble embryonic stem cells (ESCs). They hold great promise for improving the generation of genetically modified large animals. However, few porcine iPSCs (piPSCs) lines obtained currently can support development of cloned embryos. Here, we generated iPSCs from porcine adipose-derived stem cells (pADSCs), using drug-inducible expression of defined human factors (Oct4, Sox2, c-Myc and Klf4). Reprogramming of iPSCs from pADSCs was more efficient than from fibroblasts, regardless of using feeder-independent or feeder-dependent manners. By addition of Lif-2i medium containing mouse Lif, CHIR99021 and PD0325901 (Lif-2i), naïve-like piPSCs were obtained under feeder-independent and serum-free conditions. These successfully reprogrammed piPSCs were characterized by short cell cycle intervals, alkaline phosphatase (AP) staining, expression of Oct4, Sox2, Nanog, SSEA3 and SSEA4, and normal karyotypes. The resemblance of piPSCs to naïve ESCs was confirmed by their packed dome morphology, growth after single-cell dissociation, Lif-dependency, up-regulation of Stella and Eras, low expression levels of TRA-1-60, TRA-1-81 and MHC I and activation of both X chromosomes. Full reprogramming of naïve-like piPSCs was evaluated by the significant up-regulation of Lin28, Esrrb, Utf1 and Dppa5, differentiating into cell types of all three germ layers in vitro and in vivo. Furthermore, nuclear transfer embryos from naïve-like piPSCs could develop to blastocysts with improved quality. Thus, we provided an efficient protocol for generating naïve-like piPSCs from pADSCs in a feeder-independent and serum-free system with controlled regulation of exogenous genes, which may facilitate optimization of culture media and the production of transgenic pigs.

Indexed as

Adipose TissueAnimalsAnimals, Genetically ModifiedCells, CulturedCellular ReprogrammingHumansInduced Pluripotent Stem CellsKruppel-Like Factor 4Stem CellsSwineKruppel-Like Factor 4

Identifiers

PMID24465482
PMCPMC3896366
OpenAlexW2072019502

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.