Evidence map›Paper›PMID 24465092›Full record

ArticlePteridines2013

Use of a Pteridine Moiety to Track DNA Uptake in Cells.

Justin A Costa, Edgar Leal-Pinto, Scott C Henderson, Troy Zabel, Mary E Hawkins, Basil Hanss

Open access · hybridAbstract read
In one paragraph

Article in Pteridines, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 48% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 4 institutions in 1 country.

Justin A CostaDivision of Nephrology, Department of Medicine, Mt. Sinai School of Medicine, New York, NY.
Edgar Leal-PintoDepartment of Physiology and Biophysics, Virginia Commonwealth University Medical Center, Richmond, VA.
Scott C HendersonDepartment of Anatomy and Neurobiology, Virginia Commonwealth University Medical Center, Richmond, VA.
Troy ZabelSouth Denver Nephrology, Denver, CO.
Mary E HawkinsLaboratory of Receptor Biology and Gene Expression, NCI/NIH, Bethesda, MD.
Basil HanssDivision of Nephrology, Department of Medicine, Mt. Sinai School of Medicine, New York, NY.
Virginia Commonwealth University Medical Center · USColorado Kidney Care · USMount Sinai Hospital · USProtein Express (United States) · US

Funding

System Biology Center in New YorkP50GM071558 · NIGMS · ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI · PI ZAPH, SUSANA NEVES · 2007 to 2017
$22.8M
Teaching biomedical and pharmacological trainees to produce FAIR data for AI & ML applicationsT32GM062754 · NIGMS · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI SCHLESSINGER, AVNER · 2001 to 2023
$6.2M
Advanced Microscopy Shared Resource FacilityR24CA095823 · NCI · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI FRIEDRICH, VICTOR LOUIS · 2002 to 2006
$1.3M
Mechanisms of Nucleic Acid Uptake and The Role of cMDHR01DK065838 · NIDDK · MOUNT SINAI SCHOOL OF MEDICINE OF NYU · PI HANSS, BASIL G · 2004 to 2008
$1.3M
NCI NIH HHS R24 CA095823NIDDK NIH HHS R01 DK065838NIGMS NIH HHS P50 GM071558NIGMS NIH HHS T32 GM062754
6 · The paper itself

Abstract

Fluorescence labeled oligonucleotides have a long history of being used to monitor nucleic acid transport and uptake. However, it is not known if the fluorescent moiety itself physically limits the number of pathways that can be used by the cell due to steric, hydrophobic, or other chemical characteristics. Here, we report a method for comparing the uptake kinetics of oligonucleotides labeled either with the fluorescent pteridine, 3-methyl-8-(2-deoxy-β-D-ribofuranosyl) isoxanthopterin (3MI), or the common fluorophore 5-carboxyfluorescein (5-FAM). We use a multiphoton microscopic technique to monitor nucleic acid uptake LLC-PK

Indexed as

DNA uptake monitoringfluroescence labelmethylisoxathopterin

Identifiers

PMID24465092
PMCPMC3898856
OpenAlexW2035662554

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.