ArticleRNA (New York, N.Y.)2014
Mechanistic comparison of Bacillus subtilis 6S-1 and 6S-2 RNAs--commonalities and differences.
Article in RNA (New York, N.Y.), 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- RIP-seq reveals RNAs that interact with RNA polymerase and primary sigma factors in bacteria.Nucleic acids research · 2024Article
- Processing and decay of 6S-1 and 6S-2 RNAs inRNA (New York, N.Y.) · 2023Article
- Probing the conformational changes ofFrontiers in molecular biosciences · 2023Article
- Involvement ofInternational journal of molecular sciences · 2022Article
- Structural and Functional Insight into the Mechanism ofNon-coding RNA · 2022Article
- Ms1 RNA Interacts With the RNA Polymerase Core inFrontiers in microbiology · 2022Article
- Insights into 6S RNA in lactic acid bacteria (LAB).BMC genomic data · 2021Article
- Effects of DNA Topology on Transcription from rRNA Promoters inMicroorganisms · 2021Article
- 6S-2 RNA deletion in the undomesticatedRNA biology · 2021Article
- Similarities and differences between 6S RNAs from Bradyrhizobium japonicum and Sinorhizobium meliloti.Journal of microbiology (Seoul, Korea) · 2020Article
- Grad-seq in a Gram-positive bacterium reveals exonucleolytic sRNA activation in competence control.The EMBO journal · 2020Article
- Characterization of 6S RNA in the Lyme disease spirochete.Molecular microbiology · 2020Article
- Intermolecular Communication inFrontiers in molecular biosciences · 2020Review
- 6S RNA, a Global Regulator of Transcription.Microbiology spectrum · 2018Review
- 6S RNA in Rhodobacter sphaeroides: 6S RNA and pRNA transcript levels peak in late exponential phase and gene deletion causes a high salt stress phenotype.RNA biology · 2017Article
- Bacillus subtilis 6S-2 RNA serves as a template for short transcripts in vivo.RNA (New York, N.Y.) · 2016Article
- Sibling rivalry: related bacterial small RNAs and their redundant and non-redundant roles.Frontiers in cellular and infection microbiology · 2014Review
- Dissemination of 6S RNA among bacteria.RNA biology · 2014Article
- Regulation of transcription by 6S RNAs: insights from the Escherichia coli and Bacillus subtilis model systems.RNA biology · 2014Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial 6S RNAs bind to the housekeeping RNA polymerase (σ(A)-RNAP in Bacillus subtilis) to regulate transcription in a growth phase-dependent manner. B. subtilis expresses two 6S RNAs, 6S-1 and 6S-2 RNA, with different expression profiles. We show in vitro that 6S-2 RNA shares hallmark features with 6S-1 RNA: Both (1) are able to serve as templates for pRNA transcription; (2) bind with comparable affinity to σ(A)-RNAP; (3) are able to specifically inhibit transcription from DNA promoters, and (4) can form stable 6S RNA:pRNA hybrid structures that (5) abolish binding to σ(A)-RNAP. However, pRNAs of equal length dissociate faster from 6S-2 than 6S-1 RNA, owing to the higher A,U-content of 6S-2 pRNAs. This could have two mechanistic implications: (1) Short 6S-2 pRNAs (<10 nt) dissociate faster instead of being elongated to longer pRNAs, which could make it more difficult for 6S-2 RNA-stalled RNAP molecules to escape from the sequestration; and (2) relative to 6S-1 RNA, 6S-2 pRNAs of equal length will dissociate more rapidly from 6S-2 RNA after RNAP release, which could affect pRNA turnover or the kinetics of 6S-2 RNA binding to a new RNAP molecule. As 6S-2 pRNAs have not yet been detected in vivo, we considered that cellular RNAP release from 6S-2 RNA might occur via 6S-1 RNA displacing 6S-2 RNA from the enzyme, either in the absence of pRNA transcription or upon synthesis of very short 6S-2 pRNAs (∼ 5-mers, which would escape detection by deep sequencing). However, binding competition experiments argued against these possibilities.
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Registered trials
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