ArticleJournal of bacteriology2014
Influence of O polysaccharides on biofilm development and outer membrane vesicle biogenesis in Pseudomonas aeruginosa PAO1.
Article in Journal of bacteriology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
73 citing papers in PubMed, 127 citations in OpenAlex.
- Targeting Outer Membrane Vesicles ofJournal of microbiology and biotechnology · 2026Review
- Article
- Helicobacter pylori-derived outer membrane vesicles: Pathogenic roles, microbiota interactions, and biomedical applications.Journal of advanced research · 2026Review
- Outer membrane vesicles: Versatile nanocarriers for therapeutic delivery and immune modulation.Acta pharmaceutica Sinica. B · 2026Review
- Surface Interactions between an Eco-Friendly Antifouling Agent andACS applied bio materials · 2026Article
- Mechanism and Molecular Design Principles of Cationic Surfactants: From Charge-Driven Membrane Interactions to Next-Generation Quaternary Ammonium Compounds.ChemMedChem · 2026Review
- Virulent strains of Pseudomonas aeruginosa are more disruptive during invasion of a microbial model community.FEMS microbiology letters · 2026Article
- Bacterial extracellular vesicles as players in biofilm dynamics and community interactions.FEMS microbiology reviews · 2026Review
- Cross-membrane cooperation among bacteria can facilitate intracellular pathogenesis.Nature communications · 2025Article
- Pseudomonas aeruginosa biofilm: treatment strategies to combat infection.Archives of microbiology · 2025Review
- Review on bacterial outer membrane vesicles: structure, vesicle formation, separation and biotechnological applications.Microbial cell factories · 2025Review
- Phage-phage competition and biofilms affect interactions between two virulent bacteriophages and Pseudomonas aeruginosa.The ISME journal · 2025Article
- Outer membrane vesicles as a platform for the discovery of antibodies to bacterial pathogens.Applied microbiology and biotechnology · 2024Review
- Bacterial membrane vesicles: orchestrators of interkingdom interactions in microbial communities for environmental adaptation and pathogenic dynamics.Frontiers in immunology · 2024Review
- Nutrient Limitation SensitizesACS infectious diseases · 2023Article
- Environmental and Taxonomic Drivers of Bacterial Extracellular Vesicle Production in Marine Ecosystems.Applied and environmental microbiology · 2023Article
- Comparative electrokinetic properties of extracellular vesicles produced by yeast and bacteria.Colloids and surfaces. B, Biointerfaces · 2023Article
- Bacterial extracellular vesicle applications in cancer immunotherapy.Bioactive materials · 2023Article
- Identification of genes involved in enhanced membrane vesicle formation inFrontiers in microbiology · 2023Article
- Outer Membrane Vesicles: Biogenesis, Functions, and Issues.Microbiology and molecular biology reviews : MMBR · 2022Review
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Pseudomonas aeruginosa is a common opportunistic human pathogen known for its ability to adapt to changes in its environment during the course of infection. These adaptations include changes in the expression of cell surface lipopolysaccharide (LPS), biofilm development, and the production of a protective extracellular exopolysaccharide matrix. Outer membrane vesicles (OMVs) have been identified as an important component of the extracellular matrix of P. aeruginosa biofilms and are thought to contribute to the development and fitness of these bacterial communities. The goal of this study was to examine the relationships between changes in the cell surface expression of LPS O polysaccharides, biofilm development, and OMV biogenesis in P. aeruginosa. We compared wild-type P. aeruginosa PAO1 with three chromosomal knockouts. These knockouts have deletions in the rmd, wbpM, and wbpL genes that produce changes in the expression of common polysaccharide antigen (CPA), O-specific antigen (OSA), or both. Our results demonstrate that changes in O polysaccharide expression do not significantly influence OMV production but do affect the size and protein content of OMVs derived from both CPA(-) and OSA(-) cells; these mutant cells also exhibited different physical properties from wild-type cells. We further examined biofilm growth of the mutants and determined that CPA(-) cells could not develop into robust biofilms and exhibit changes in cell morphology and biofilm matrix production. Together these results demonstrate the importance of O polysaccharide expression on P. aeruginosa OMV composition and highlight the significance of CPA expression in biofilm development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.