ArticleApplied and environmental microbiology2014
Whole-genome single-nucleotide-polymorphism analysis for discrimination of Clostridium botulinum group I strains.
Article in Applied and environmental microbiology, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 32 citations in OpenAlex.
- Foodborne Botulism Caused byMicroorganisms · 2025Article
- Closed genome sequence ofMicrobiology resource announcements · 2024Article
- Genomic Diversity, Competition, and Toxin Production by Group I and IIMicroorganisms · 2022Article
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- Current Developments in Diagnostic Assays for Laboratory Confirmation and Investigation of Botulism.Journal of clinical microbiology · 2022Review
- Genomic Characterization of Strains From a Cluster of Infant Botulism Type A in a Small Town in Colorado, United States.Frontiers in microbiology · 2021Article
- Article
- ClosingFrontiers in microbiology · 2020Article
- Closed Genome Sequences of Two Clostridium botulinum Strains Obtained by Nanopore Sequencing.Microbiology resource announcements · 2018Article
- Botulinum Neurotoxin Detection Methods for Public Health Response and Surveillance.Frontiers in bioengineering and biotechnology · 2018Review
- Defining a Core Genome Multilocus Sequence Typing Scheme for the Global Epidemiology of Vibrio parahaemolyticus.Journal of clinical microbiology · 2017Article
- Finished Whole-Genome Sequences of TwoGenome announcements · 2017Article
- Impact ofCurrent opinion in food science · 2016Review
- Diversity of Group I and II Clostridium botulinum Strains from France Including Recently Identified Subtypes.Genome biology and evolution · 2016Article
- A Nonautochthonous U.S. Strain of Vibrio parahaemolyticus Isolated from Chesapeake Bay Oysters Caused the Outbreak in Maryland in 2010.Applied and environmental microbiology · 2016Article
- Comparative genomic analyses reveal broad diversity in botulinum-toxin-producing Clostridia.BMC genomics · 2016Article
- Genomic Epidemiology of Clostridium botulinum Isolates from Temporally Related Cases of Infant Botulism in New South Wales, Australia.Journal of clinical microbiology · 2015Article
- Clostridium botulinum Group II Isolate Phylogenomic Profiling Using Whole-Genome Sequence Data.Applied and environmental microbiology · 2015Article
- Historical and current perspectives on Clostridium botulinum diversity.Research in microbiology · 2015Review
- Genomic sequences of six botulinum neurotoxin-producing strains representing three clostridial species illustrate the mobility and diversity of botulinum neurotoxin genes.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2015Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium botulinum is a genetically diverse Gram-positive bacterium producing extremely potent neurotoxins (botulinum neurotoxins A through G [BoNT/A-G]). The complete genome sequences of three strains harboring only the BoNT/A1 nucleotide sequence are publicly available. Although these strains contain a toxin cluster (HA(+) OrfX(-)) associated with hemagglutinin genes, little is known about the genomes of subtype A1 strains (termed HA(-) OrfX(+)) that lack hemagglutinin genes in the toxin gene cluster. We sequenced the genomes of three BoNT/A1-producing C. botulinum strains: two strains with the HA(+) OrfX(-) cluster (69A and 32A) and one strain with the HA(-) OrfX(+) cluster (CDC297). Whole-genome phylogenic single-nucleotide-polymorphism (SNP) analysis of these strains along with other publicly available C. botulinum group I strains revealed five distinct lineages. Strains 69A and 32A clustered with the C. botulinum type A1 Hall group, and strain CDC297 clustered with the C. botulinum type Ba4 strain 657. This study reports the use of whole-genome SNP sequence analysis for discrimination of C. botulinum group I strains and demonstrates the utility of this analysis in quickly differentiating C. botulinum strains harboring identical toxin gene subtypes. This analysis further supports previous work showing that strains CDC297 and 657 likely evolved from a common ancestor and independently acquired separate BoNT/A1 toxin gene clusters at distinct genomic locations.
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